Wegner M (1998)
Publication Type: Journal article, Original article
Publication year: 1998
Publisher: American Society for Biochemistry and Molecular Biology
Book Volume: 273
Pages Range: 14538-14544
Journal Issue: 23
In order to search for genes that are activated by muscarinic acetylcholine receptors (mAChRs), we used an mRNA differential display approach in HEK293 cells expressing m1AChR. The zinc-finger transcription factor genes Egr-1, Egr-2, and Egr-3 were identified. Northern blot analyses confirmed that mRNA levels of Egr-1, Egr-2, and Egr-3 increased readily after mAChR stimulation and that a maximum was attained within 50 min. At that time, Egr-4 mRNA was also detectable. Western blots and electromobility shift assays demonstrated synthesis of EGR-1 and EGR-3, as well as binding to DNA recognition sites in response to m1AChR activation. Activation of m1AChR increased transcription from EGR-dependent promoters, including the acetylcholinesterase gene promoter. Activity-dependent regulation of Egr-1 mRNA expression and EGR-1 protein synthesis was also observed in cells expressing m2, m3, or m4AChR subtypes. Increased EGR-1 synthesis was mimicked by phorbol myristate acetate, but not by forskolin, and receptor-stimulated EGR-1 synthesis was partially inhibited by phorbol myristate acetate down- regulation. Together, our results demonstrate that muscarinic receptor signaling activates the EGR transcription factor family and that PKC may be involved in intracellular signaling. The data suggest that transcription of EGR-dependent target genes, including the AChE gene, can be under the control of extracellular and intracellular signals coupled to muscarinic receptors.
APA:
Wegner, M. (1998). Muscarinic acetylcholine receptors activate expression of the EGr gene family of transcription factors. Journal of Biological Chemistry, 273(23), 14538-14544. https://doi.org/10.1074/jbc.273.23.14538
MLA:
Wegner, Michael. "Muscarinic acetylcholine receptors activate expression of the EGr gene family of transcription factors." Journal of Biological Chemistry 273.23 (1998): 14538-14544.
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