Novel strontium-doped bioactive glass nanoparticles enhance proliferation and osteogenic differentiation of human bone marrow stromal cells

Strobel L, Hild N, Mohn D, Stark WJ, Hoppe A, Gbureck U, Horch RE, Kneser U, Boccaccini AR (2013)

Publication Type: Journal article

Publication year: 2013

Journal

Publisher: Springer Verlag (Germany)

Book Volume: 15

Pages Range: 1780

Journal Issue: 7

DOI: 10.1007/s11051-013-1780-5

Abstract

The present study investigates a new family of bioactive glass nanoparticles with and without Sr-doping focusing on the influence of the nanoparticles on human bone marrow stromal cells (hBMSCs) in vitro. The bioactive glass nanoparticles were fabricated by flame spray synthesis and a particle diameter of 30-35 nm was achieved. Glass nanoparticles were undoped (BG 13-93-0Sr) or doped with 5 wt% strontium (Sr) (BG 13-93-5Sr) and used at concentrations of 10 and 100 μg/cm (particles per culture plate area), respectively. Cells were cultured for 14 days after which the samples were analysed regarding metabolic activity and expression of various bone-specific genes. Cell growth and morphology indicated the high cytocompatibility of the nanoparticulate bioactive glass. The presence of the nanoparticles enhanced cell growth compared to the plain polystyrene control group. At a concentration of 100 μg/cm, Sr-doped particles led to significantly enhanced gene expression of osteocalcin, collagen type 1 and vascular endothelial growth factor. Thus, Sr-doped nanoparticles showing a dose-dependent increase of osteogenic differentiation in hBMSCs are a promising biomaterial for bone regeneration purposes. © 2013 Springer Science+Business Media Dordrecht.

Authors with CRIS profile

Involved external institutions

Eidgenössische Technische Hochschule Zürich (ETHZ) / Swiss Federal Institute of Technology in Zurich Switzerland (CH) Julius-Maximilians-Universität Würzburg Germany (DE)

How to cite

APA:

Strobel, L., Hild, N., Mohn, D., Stark, W.J., Hoppe, A., Gbureck, U.,... Boccaccini, A.R. (2013). Novel strontium-doped bioactive glass nanoparticles enhance proliferation and osteogenic differentiation of human bone marrow stromal cells. Journal of Nanoparticle Research, 15(7), 1780. https://dx.doi.org/10.1007/s11051-013-1780-5

MLA:

Strobel, Leonie, et al. "Novel strontium-doped bioactive glass nanoparticles enhance proliferation and osteogenic differentiation of human bone marrow stromal cells." Journal of Nanoparticle Research 15.7 (2013): 1780.

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