IgG subclass and vaccination stimulus determine changes in antigen specific antibody glycosylation in mice
Beitrag in einer Fachzeitschrift
Details zur Publikation
Autor(en): Kao D, Lux A, Schaffert A, Lang R, Altmann F, Nimmerjahn F
Zeitschrift: → European Journal of Immunology |
Jahr der Veröffentlichung: 2017
ISSN: 0014-2980
Abstract
Immunoglobulin G (IgG) glycosylation can modulate antibody effector functions. Depending on the precise composition of the sugar moiety attached to individual IgG glycovariants either pro- or anti-inflammatory effector pathways can be initiated via differential binding to type I or type II Fc-receptors. However, an in depth understanding of how individual IgG subclasses are glycosylated during the steady state and how their glycosylation pattern changes during vaccination is missing. To monitor IgG subclass glycosylation during the steady state and upon vaccination of mice with different T-cell dependent and independent antigens, tryptic digests of serum, and antigen-specific IgG preparations were analyzed by reversed phase-liquid chromatography-mass spectrometry. We show that there is a remarkable difference with respect to how individual IgG subclasses are glycosylated during the steady state. More importantly, upon T-cell dependent and independent vaccinations, individual antigen-specific IgG subclasses reacted differently with respect to changes in individual glycoforms, suggesting that the IgG subclass itself is a major determinant of restricting or allowing alterations in specific IgG glycovariants.
FAU-Autoren / FAU-Herausgeber
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| | | Professur für Angeborene Immunität und Pathogenerkennung |
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| Lux, Anja Dr. | Nimmerjahn, Falk Prof. Dr. | |
| | Lehrstuhl für Genetik | Lehrstuhl für Genetik | |
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Autor(en) der externen Einrichtung(en)
→ Universität für Bodenkultur Wien (BOKU( / University of Natural Resources and Life Sciences, Vienna |
Zitierweisen
APA: | Kao, D., Lux, A., Schaffert, A., Lang, R., Altmann, F., & Nimmerjahn, F. (2017). IgG subclass and vaccination stimulus determine changes in antigen specific antibody glycosylation in mice. European Journal of Immunology. https://dx.doi.org/10.1002/eji.201747208 |
MLA: | Kao, Daniela, et al. "IgG subclass and vaccination stimulus determine changes in antigen specific antibody glycosylation in mice." European Journal of Immunology (2017). |