IgG subclass and vaccination stimulus determine changes in antigen specific antibody glycosylation in mice

Beitrag in einer Fachzeitschrift


Details zur Publikation

Autor(en): Kao D, Lux A, Schaffert A, Lang R, Altmann F, Nimmerjahn F
Zeitschrift: European Journal of Immunology
Jahr der Veröffentlichung: 2017
ISSN: 0014-2980


Abstract


Immunoglobulin G (IgG) glycosylation can modulate antibody effector functions. Depending on the precise composition of the sugar moiety attached to individual IgG glycovariants either pro- or anti-inflammatory effector pathways can be initiated via differential binding to type I or type II Fc-receptors. However, an in depth understanding of how individual IgG subclasses are glycosylated during the steady state and how their glycosylation pattern changes during vaccination is missing. To monitor IgG subclass glycosylation during the steady state and upon vaccination of mice with different T-cell dependent and independent antigens, tryptic digests of serum, and antigen-specific IgG preparations were analyzed by reversed phase-liquid chromatography-mass spectrometry. We show that there is a remarkable difference with respect to how individual IgG subclasses are glycosylated during the steady state. More importantly, upon T-cell dependent and independent vaccinations, individual antigen-specific IgG subclasses reacted differently with respect to changes in individual glycoforms, suggesting that the IgG subclass itself is a major determinant of restricting or allowing alterations in specific IgG glycovariants.



FAU-Autoren / FAU-Herausgeber

Kao, Daniela
Lehrstuhl für Genetik
Lang, Roland Prof. Dr.
Professur für Angeborene Immunität und Pathogenerkennung
Lux, Anja Dr.
Lehrstuhl für Genetik
Nimmerjahn, Falk Prof. Dr.
Lehrstuhl für Genetik
Schaffert, Anja
Lehrstuhl für Genetik


Autor(en) der externen Einrichtung(en)
Universität für Bodenkultur Wien (BOKU( / University of Natural Resources and Life Sciences, Vienna


Zitierweisen

APA:
Kao, D., Lux, A., Schaffert, A., Lang, R., Altmann, F., & Nimmerjahn, F. (2017). IgG subclass and vaccination stimulus determine changes in antigen specific antibody glycosylation in mice. European Journal of Immunology. https://dx.doi.org/10.1002/eji.201747208

MLA:
Kao, Daniela, et al. "IgG subclass and vaccination stimulus determine changes in antigen specific antibody glycosylation in mice." European Journal of Immunology (2017).

BibTeX: 

Zuletzt aktualisiert 2018-21-11 um 06:02