Olig2 regulates Sox10 expression in oligodendrocyte precursors through an evolutionary conserved distal enhancer.
Küspert M, Hammer A, Bösl M, Wegner M (2011)
Publication Type: Journal article
Publication year: 2011
Journal
Publisher: Oxford University Press (OUP): Policy C - Option B / Oxford University Press
Pages Range: 1280-1293
Journal Issue: 39
DOI: 10.1093/nar/gkq951
Abstract
The HMG-domain transcription factor Sox10 is expressed throughout oligodendrocyte development and is an important component of the transcriptional regulatory network in these myelin-forming CNS glia. Of the known Sox10 regulatory regions, only the evolutionary conserved U2 enhancer in the distal 5′-flank of the Sox10 gene exhibits oligodendroglial activity. We found that U2 was active in oligodendrocyte precursors, but not in mature oligodendrocytes. U2 activity also did not mediate the initial Sox10 induction after specification arguing that Sox10 expression during oligodendroglial development depends on the activity of multiple regulatory regions. The oligodendroglial bHLH transcription factor Olig2, but not the closely related Olig1 efficiently activated the U2 enhancer. Olig2 bound U2 directly at several sites including a highly conserved one in the U2 core. Inactivation of this site abolished the oligodendroglial activity of U2 in vivo. In contrast to Olig2, the homeodomain transcription factor Nkx6.2 repressed U2 activity. Repression may involve recruitment of Nkx6.2 to U2 and inactivation of Olig2 and other activators by protein-protein interactions. Considering the selective expression of Nkx6.2 at the time of specification and in differentiated oligodendrocytes, Nkx6.2 may be involved in limiting U2 activity to the precursor stage during oligodendrocyte development. The Author(s) 2010. Published by Oxford University Press.2010This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/ licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. © The Author(s) 2010.
Authors with CRIS profile
Melanie Küspert
Lehrstuhl für Biochemie und Pathobiochemie
Michael Wegner
Lehrstuhl für Biochemie und Pathobiochemie
Involved external institutions
Germany (DE)How to cite
APA:
Küspert, M., Hammer, A., Bösl, M., & Wegner, M. (2011). Olig2 regulates Sox10 expression in oligodendrocyte precursors through an evolutionary conserved distal enhancer. Nucleic Acids Research, 39, 1280-1293. https://doi.org/10.1093/nar/gkq951
MLA:
Küspert, Melanie, et al. "Olig2 regulates Sox10 expression in oligodendrocyte precursors through an evolutionary conserved distal enhancer." Nucleic Acids Research 39 (2011): 1280-1293.
BibTeX: Download