The influence of tetracycline loading on the surface morphology and biocompatibility of films made from P(3HB) microspheres

Francis L, Meng D, Locke IC, Mordan N, Salih V, Knowles JC, Boccaccini AR, Roy I (2010)

Publication Type: Journal article

Publication year: 2010

Journal

Advanced Engineering Materials Wiley-VCH Verlag

Publisher: Wiley-VCH Verlag

Book Volume: 12

Pages Range: B260-B268

DOI: 10.1002/adem.201080020

Abstract

Tetracycline, an antibiotic used against a broad range of Gram positive and Gram negative bacteria was encapsulated in microspheres made of poly(3-hydroxybutyric acid) P(3HB), a microbial biodegradable polymer isolated from Bacillus cereus SPV. The drug loaded microspheres were prepared using an oil emulsion technique and compressed uniaxially to produce films. Although the same fabrication conditions were used for preparing the drug loaded and unloaded microspheres, the presence of the drug changed the surface morphology and roughness of the films. The surface morphology of the drug loaded films appeared uneven and coarser and the roughness, with an average root mean square value of 5.89mm, was significantly higher than that of the unloaded film. The in vitro biocompatibility of the films was investigated using a human keratinocyte cell line (HaCaT) by comparing cell viability on the films to that on conventional tissue culture plastics. Both films appear to support cell growth but cell attachment and percentage cell viability were greater on the drug loaded films (32% of control) compared to the unloaded film (10% of control), possibly as a result of the non-uniform surface morphology and increased roughness of the drug loaded film. Thus, the above results illustrate that the drug loaded films, in addition to being a suitable matrix for drug delivery, represent an improved substrate for keratinocyte cell attachment. © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Authors with CRIS profile

Aldo Boccaccini Lehrstuhl für Werkstoffwissenschaften (Biomaterialien)

Involved external institutions

University College London (UCL) GB United Kingdom (GB) University of Westminster GB United Kingdom (GB) Imperial College London / The Imperial College of Science, Technology and Medicine GB United Kingdom (GB)

How to cite

APA:

Francis, L., Meng, D., Locke, I.C., Mordan, N., Salih, V., Knowles, J.C.,... Roy, I. (2010). The influence of tetracycline loading on the surface morphology and biocompatibility of films made from P(3HB) microspheres. Advanced Engineering Materials, 12, B260-B268. https://doi.org/10.1002/adem.201080020

MLA:

Francis, Lydia, et al. "The influence of tetracycline loading on the surface morphology and biocompatibility of films made from P(3HB) microspheres." Advanced Engineering Materials 12 (2010): B260-B268.

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