Rapid purification of a functionally active plant sucrose carrier from transgenic yeast using a bacterial biotin acceptor domain.
Stolz J, Sauer N, Darnhofer-Demar B (1995)
Publication Language: English
Publication Status: Published
Publication Type: Journal article, Original article
Publication year: 1995
Journal
Book Volume: 377
Pages Range: 167-71
Journal Issue: 2
DOI: 10.1016/0014-5793(95)01333-4
Open Access Link: http://onlinelibrary.wiley.com/doi/10.1016/0014-5793(95)01333-4/abstract
Abstract
A rapid and efficient method has been used for the purification of a Plantago major sucrose carrier from Saccharomyces cerevisiae. The C-terminal fusion of a bacterial biotin acceptor domain to the carrier protein did not interfere with the targeting to the yeast plasma membrane nor with the catalytic activity of the sucrose carrier. The chimeric construct is biotinylated by yeast cells in vivo and represents the only biotinylated protein in yeast membranes. Solubilized biotinylated carrier protein binds selectively to immobilized monomeric avidin and can be eluted as pure protein with free biotin. The purified protein is functionally active and catalyzes the energy-dependent transport of sucrose into proteoliposomes.
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Germany (DE)How to cite
APA:
Stolz, J., Sauer, N., & Darnhofer-Demar, B. (1995). Rapid purification of a functionally active plant sucrose carrier from transgenic yeast using a bacterial biotin acceptor domain. Febs Letters, 377(2), 167-71. https://doi.org/10.1016/0014-5793(95)01333-4
MLA:
Stolz, Jürgen, Norbert Sauer, and Brigitte Darnhofer-Demar. "Rapid purification of a functionally active plant sucrose carrier from transgenic yeast using a bacterial biotin acceptor domain." Febs Letters 377.2 (1995): 167-71.
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