Biomimetic poly(glycerol sebacate) (PGS) membranes for cardiac patch application

Rai R, Tallawi M, Barbani N, Frati C, Madeddu D, Cavali S, Graiani G, Quaini F, Schubert DW, Roether J, Rosellini E, Boccaccini AR (2013)

Publication Language: English

Publication Type: Journal article, Review article

Publication year: 2013

Journal

Materials Science and Engineering C Elsevier

Publisher: Elsevier

Book Volume: 33

Pages Range: 3677–3687

Journal Issue: 7

DOI: 10.1016/j.msec.2013.04.058

Abstract

In this study biomimetic poly(glycerol sebacate) PGS matrix was developed for cardiac patch application. The rationale was that such matrices would provide conducive environment for the seeded cells at the interphase with PGS. From the microstructural standpoint, PGS was fabricated into dense films and porous PGS scaffolds. From the biological aspect, biomimetic PGS membranes were developed via covalently binding peptides Tyr-Ile-Gly-Ser-Arg (YIGSR) and Gly-Arg-Gly-Asp-Ser-Pro (GRGDSP), corresponding to the epitope sequences of laminin and fibronectin, respectively onto the surface. To improve and enhance homogenous binding of peptides onto the PGS surface, chemical modification of its surface was carried out. A sequential regime of alkaline hydrolysis with 0.01 M NaOH for 5 min and acidification with 0.01 M HCl for 25 s was optimal. More COOH chemical group was exposed without causing deleterious effect on the bulk properties of the polymer as revealed by the physicochemical analysis carried out. HPLC analysis, chemical imaging and ToF-SIMS were able to establish the successful homogenous functionalization of PGS membranes with the peptides. Finally, the developed biomimetic membranes supported the adhesion and growth of rat and human cardiac progenitor cells.

Authors with CRIS profile

Ranjana Rai Lehrstuhl für Werkstoffwissenschaften (Biomaterialien) Marwa Tallawi Lehrstuhl für Werkstoffwissenschaften (Biomaterialien) Dirk Schubert Lehrstuhl für Werkstoffwissenschaften (Polymerwerkstoffe) Judith Roether Lehrstuhl für Werkstoffwissenschaften (Polymerwerkstoffe) Aldo Boccaccini Lehrstuhl für Werkstoffwissenschaften (Biomaterialien)

Involved external institutions

University of Parma / Università degli Studi di Parma IT Italy (IT) University of Pisa / Università di Pisa (UniPi) IT Italy (IT)

How to cite

APA:

Rai, R., Tallawi, M., Barbani, N., Frati, C., Madeddu, D., Cavali, S.,... Boccaccini, A.R. (2013). Biomimetic poly(glycerol sebacate) (PGS) membranes for cardiac patch application. Materials Science and Engineering C, 33(7), 3677–3687. https://doi.org/10.1016/j.msec.2013.04.058

MLA:

Rai, Ranjana, et al. "Biomimetic poly(glycerol sebacate) (PGS) membranes for cardiac patch application." Materials Science and Engineering C 33.7 (2013): 3677–3687.

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