Beyond endoscopic assessment in inflammatory bowel disease: real-time histology of disease activity by non-linear multimodal imaging.

Beitrag in einer Fachzeitschrift


Details zur Publikation

Autorinnen und Autoren: Chernavskaia O, Heuke S, Vieth M, Friedrich O, Schürmann S, Atreya R, Stallmach A, Neurath M, Waldner M, Petersen I, Schmitt M, Bocklitz T, Popp J
Zeitschrift: Scientific Reports
Verlag: Nature Publishing Group: Open Access Journals - Option B
Jahr der Veröffentlichung: 2016
Band: 6
Seitenbereich: 29239
ISSN: 2045-2322
Sprache: Englisch


Abstract


Assessing disease activity is a prerequisite for an adequate treatment of inflammatory bowel diseases (IBD) such as Crohn's disease and ulcerative colitis. In addition to endoscopic mucosal healing, histologic remission poses a promising end-point of IBD therapy. However, evaluating histological remission harbors the risk for complications due to the acquisition of biopsies and results in a delay of diagnosis because of tissue processing procedures. In this regard, non-linear multimodal imaging techniques might serve as an unparalleled technique that allows the real-time evaluation of microscopic IBD activity in the endoscopy unit. In this study, tissue sections were investigated using the non-linear multimodal microscopy combination of coherent anti-Stokes Raman scattering (CARS), two-photon excited auto fluorescence (TPEF) and second-harmonic generation (SHG). After the measurement a gold-standard assessment of histological indexes was carried out based on a conventional H&E stain. Subsequently, various geometry and intensity related features were extracted from the multimodal images. An optimized feature set was utilized to predict histological index levels based on a linear classifier. Based on the automated prediction, the diagnosis time interval is decreased. Therefore, non-linear multimodal imaging may provide a real-time diagnosis of IBD activity suited to assist clinical decision making within the endoscopy unit.



FAU-Autorinnen und Autoren / FAU-Herausgeberinnen und Herausgeber

Atreya, Raja Prof. Dr.
Professur für Translationale Immunforschung bei chronisch entzündlichen Darmerkrankungen (Heisenberg-Professur)
Friedrich, Oliver Prof. Dr. Dr.
Lehrstuhl für Medizinische Biotechnologie
Neurath, Markus Prof. Dr.
Lehrstuhl für Innere Medizin I
Schürmann, Sebastian Dr. rer. nat.
Lehrstuhl für Medizinische Biotechnologie


Zusätzliche Organisationseinheit(en)
Erlangen Graduate School in Advanced Optical Technologies


Einrichtungen weiterer Autorinnen und Autoren

Friedrich-Schiller-Universität Jena
Klinikum Bayreuth
Leibniz-Institut für Photonische Technologien e.V.
Universitätsklinikum Jena


Zitierweisen

APA:
Chernavskaia, O., Heuke, S., Vieth, M., Friedrich, O., Schürmann, S., Atreya, R.,... Popp, J. (2016). Beyond endoscopic assessment in inflammatory bowel disease: real-time histology of disease activity by non-linear multimodal imaging. Scientific Reports, 6, 29239. https://dx.doi.org/10.1038/srep29239

MLA:
Chernavskaia, Olga, et al. "Beyond endoscopic assessment in inflammatory bowel disease: real-time histology of disease activity by non-linear multimodal imaging." Scientific Reports 6 (2016): 29239.

BibTeX: 

Zuletzt aktualisiert 2019-28-02 um 06:06