Second harmonic generation microscopy probes different states of motor protein interaction in myofibrils
Schürmann S, von Wegner F, Fink R, Friedrich O, Vogel M (2010)
Publication Language: English
Publication Type: Journal article, Original article
Publication year: 2010
Journal
Original Authors: Schurmann S., Von Wegner F., Fink R.H.A., Friedrich O., Vogel M.
Publisher: Elsevier (Cell Press) / Biophysical Society
Book Volume: 99
Pages Range: 1842-1851
Journal Issue: 6
DOI: 10.1016/j.bpj.2010.07.005
Abstract
The second harmonic generation (SHG) signal intensity sourced from skeletal muscle myosin II strongly depends on the polarization of the incident laser beam relative to the muscle fiber axis. This dependence is related to the second-order susceptibility x(2), which can be described by a single component ratio γ under generally assumed symmetries. We precisely extracted γ from SHG polarization dependence curves with an extended focal field model. In murine myofibrillar preparations, we have found two distinct polarization dependencies: With the actomyosin system in the rigor state, γ . has a mean value of γ = 0.52 (SD = 0.04, n = 55); in a relaxed state where myosin is not bound to actin, γ has a mean value of γ = 0.24 (SD = 0.07, n = 70). We observed a similar value in an activated state where the myosin power stroke was pharmacologically inhibited using N-benzyl-p-toluene sulfonamide. In summary, different actomyosin states can be visualized noninvasively with SHG microscopy. Specifically, SHG even allows us to distinguish different actin-bound states of myosin II using γ as a parameter. © 2010 by the Biophysical Society.
Authors with CRIS profile
Sebastian Schürmann
Lehrstuhl für Medizinische Biotechnologie
Oliver Friedrich
Lehrstuhl für Medizinische Biotechnologie
Involved external institutions
Ruprecht-Karls-Universität Heidelberg
Germany (DE)
Harvard University
United States (USA) (US)
Goethe-Universität Frankfurt am Main
Germany (DE)
Germany (DE)
Harvard University
United States (USA) (US)
Goethe-Universität Frankfurt am Main
Germany (DE)
How to cite
APA:
Schürmann, S., von Wegner, F., Fink, R., Friedrich, O., & Vogel, M. (2010). Second harmonic generation microscopy probes different states of motor protein interaction in myofibrils. Biophysical Journal, 99(6), 1842-1851. https://dx.doi.org/10.1016/j.bpj.2010.07.005
MLA:
Schürmann, Sebastian, et al. "Second harmonic generation microscopy probes different states of motor protein interaction in myofibrils." Biophysical Journal 99.6 (2010): 1842-1851.
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