Genotoxicity of Superparamagnetic Iron Oxide Nanoparticles in Granulosa Cells

Poettler M, Staicu A, Zaloga J, Unterweger H, Weigel B, Schreiber E, Hofmann S, Wiest I, Jeschke U, Alexiou C, Janko C (2015)

Publication Type: Journal article

Publication year: 2015

Journal

International Journal of Molecular Sciences MDPI

Book Volume: 16

Pages Range: 26280-90

Journal Issue: 11

DOI: 10.3390/ijms161125960

Abstract

Nanoparticles that are aimed at targeting cancer cells, but sparing healthy tissue provide an attractive platform of implementation for hyperthermia or as carriers of chemotherapeutics. According to the literature, diverse effects of nanoparticles relating to mammalian reproductive tissue are described. To address the impact of nanoparticles on cyto- and genotoxicity concerning the reproductive system, we examined the effect of superparamagnetic iron oxide nanoparticles (SPIONs) on granulosa cells, which are very important for ovarian function and female fertility. Human granulosa cells (HLG-5) were treated with SPIONs, either coated with lauric acid (SEONLA) only, or additionally with a protein corona of bovine serum albumin (BSA; SEON(LA-BSA)), or with dextran (SEON(DEX)). Both micronuclei testing and the detection of ?H2A.X revealed no genotoxic effects of SEON(LA-BSA), SEON(DEX) or SEON(LA). Thus, it was demonstrated that different coatings of SPIONs improve biocompatibility, especially in terms of genotoxicity towards cells of the reproductive system.

Authors with CRIS profile

Jan Zaloga Department of Otorhinolaryngology – Head and Neck Surgery (HNO-Klinik) Harald Unterweger Department of Otorhinolaryngology – Head and Neck Surgery (HNO-Klinik) Christoph Alexiou Professur für Nanomedizin Christina Janko Department of Otorhinolaryngology – Head and Neck Surgery (HNO-Klinik)

Additional Organisation(s)

Exzellenz-Cluster Engineering of Advanced Materials (EAM)

Involved external institutions

Ludwig-Maximilians-Universität (LMU) DE Germany (DE)

How to cite

APA:

Poettler, M., Staicu, A., Zaloga, J., Unterweger, H., Weigel, B., Schreiber, E.,... Janko, C. (2015). Genotoxicity of Superparamagnetic Iron Oxide Nanoparticles in Granulosa Cells. International Journal of Molecular Sciences, 16(11), 26280-90. https://doi.org/10.3390/ijms161125960

MLA:

Poettler, Marina, et al. "Genotoxicity of Superparamagnetic Iron Oxide Nanoparticles in Granulosa Cells." International Journal of Molecular Sciences 16.11 (2015): 26280-90.

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