Long term stability of Oligo (dT) 25 magnetic beads for the expression analysis of Euglena gracilis for long term space projects

Beitrag in einer Fachzeitschrift
(Originalarbeit)


Details zur Publikation

Autor(en): Becker I, Strauch S, Hauslage J, Lebert M
Zeitschrift: Life Sciences in Space Research
Verlag: Elsevier Ltd
Jahr der Veröffentlichung: 2017
Band: 13
Seitenbereich: 12-18
ISSN: 2214-5532


Abstract


The unicellular freshwater flagellate Euglena gracilis has a highly developed sensory system. The cells use different stimuli such as light and gravity to orient themselves in the surrounding medium to find areas for optimal growth. Due to the ability to produce oxygen and consume carbon dioxide, Euglena is a suitable candidate for life support systems. Participation in a long-term space experiment would allow for the analysis of changes and adaptations to the new environment, and this could bring new insights into the mechanism of perception of gravity and the associated signal transduction chain. For a molecular analysis of transcription patterns, an automated system is necessary, capable of performing all steps from taking a sample, processing it and generating data. One of the developmental steps is to find long-term stable reagents and materials and test them for stability at higher-than-recommended temperature conditions during extended storage time. We investigated the usability of magnetic beads in an Euglena specific lysis buffer after addition of the RNA stabilizer Dithiothreitol over 360 days and the lysis buffer with the stabilizer alone over 455 days at the expected storage temperature of 19 °C. We can claim that the stability is not impaired at all after an incubation period of over one year. This might be an interesting result for researchers who have to work under non-standard lab conditions, as in biological or medicinal fieldwork.



FAU-Autoren / FAU-Herausgeber

Becker, Ina
Lehrstuhl für Zellbiologie
Lebert, Michael PD Dr.
Lehrstuhl für Zellbiologie
Strauch, Sebastian Dr.
Lehrstuhl für Zellbiologie


Autor(en) der externen Einrichtung(en)
Deutsches Zentrum für Luft- und Raumfahrt e.V. (DLR)


Zitierweisen

APA:
Becker, I., Strauch, S., Hauslage, J., & Lebert, M. (2017). Long term stability of Oligo (dT) 25 magnetic beads for the expression analysis of Euglena gracilis for long term space projects. Life Sciences in Space Research, 13, 12-18. https://dx.doi.org/10.1016/j.lssr.2017.02.001

MLA:
Becker, Ina, et al. "Long term stability of Oligo (dT) 25 magnetic beads for the expression analysis of Euglena gracilis for long term space projects." Life Sciences in Space Research 13 (2017): 12-18.

BibTeX: 

Zuletzt aktualisiert 2018-09-11 um 13:50