Distinct functionality of dishevelled isoforms on Ca2+/calmodulin-dependent protein kinase 2 (CamKII) in Xenopus gastrulation.

Beitrag in einer Fachzeitschrift
(Originalarbeit)


Details zur Publikation

Autorinnen und Autoren: Gentzel M, Schille C, Rauschenberger V, Schambony A
Zeitschrift: Molecular Biology of the Cell
Jahr der Veröffentlichung: 2015
Band: 26
Heftnummer: 5
Seitenbereich: 966-977
ISSN: 1059-1524


Abstract


Wnt ligands trigger the activation of a variety of β-catenin-dependent and β-catenin-independent intracellular signaling cascades. Despite the variations in intracellular signaling, Wnt pathways share the effector proteins frizzled, dishevelled, and β-arrestin. It is unclear how the specific activation of individual branches and the integration of multiple signals are achieved. We hypothesized that the composition of dishevelled-β-arrestin protein complexes contributes to signal specificity and identified CamKII as an interaction partner of the dishevelled-β-arrestin protein complex by quantitative functional proteomics. Specifically, we found that CamKII isoforms interact differentially with the three vertebrate dishevelled proteins. Dvl1 is required for the activation of CamKII and PKC in the Wnt/Ca(2+) pathway. However, CamKII interacts with Dvl2 but not with Dvl1, and Dvl2 is necessary to mediate CamKII function downstream of Dvl1 in convergent extension movements in Xenopus gastrulation. Our findings indicate that the different Dvl proteins and the composition of dishevelled-β-arrestin protein complexes contribute to the specific activation of individual branches of Wnt signaling.



FAU-Autorinnen und Autoren / FAU-Herausgeberinnen und Herausgeber

Rauschenberger, Verena
Lehrstuhl für Entwicklungsbiologie
Schambony, Alexandra Prof. Dr.
Lehrstuhl für Entwicklungsbiologie
Schille, Carolin
Lehrstuhl für Entwicklungsbiologie


Einrichtungen weiterer Autorinnen und Autoren

Max-Planck-Institut für molekulare Zellbiologie und Genetik / Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)


Zitierweisen

APA:
Gentzel, M., Schille, C., Rauschenberger, V., & Schambony, A. (2015). Distinct functionality of dishevelled isoforms on Ca2+/calmodulin-dependent protein kinase 2 (CamKII) in Xenopus gastrulation. Molecular Biology of the Cell, 26(5), 966-977. https://dx.doi.org/10.1091/mbc.E14-06-1089

MLA:
Gentzel, Marc, et al. "Distinct functionality of dishevelled isoforms on Ca2+/calmodulin-dependent protein kinase 2 (CamKII) in Xenopus gastrulation." Molecular Biology of the Cell 26.5 (2015): 966-977.

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Zuletzt aktualisiert 2018-10-08 um 15:53