Detsch R (2010)
Publication Status: Published
Publication Type: Journal article, Original article
Publication year: 2010
Publisher: Elsevier
Book Volume: 38
Pages Range: 423-430
Journal Issue: 6
DOI: 10.1016/j.jcms.2010.01.003
Introduction: Angiogenesis and mesenchymal stem cells (MSCs) promote osteogenesis. The aim of the present study was to evaluate whether bone morphogenetic protein (BMP-7) promoted osteoinduction could be enhanced by combining it with vascular endothelial growth factor (VEGF) or MSCs in highly porous biphasic calcium phosphate (BCP) ceramics. Materials and methods: BCP ceramic blocks were implanted in an ectopic site in 24 mice (BMP-7 vs. BMP-7/VEGF; BMP-7 vs. BMP-7/MSCs and BMP-7 vs. Control; each group n = 8). Specimens were analysed 12 weeks after surgery by environmental scanning electron microscopy (ESEM) and Giemsa staining. Results: In all implanted scaffolds, newly formed bone was observed, even in the control site. No statistical differences in the amount of new bone were found in the presence of BMP-7 compared to BMP-7/VEGF (p = 1.0) or BMP-7/MSCs (p = 0.786). ESEM revealed a degradation of the scaffolds. A higher degradation was observed in areas where no bone-implant contact was present compared to areas where the ceramic was integrated in newly formed bone. Conclusions: Neither VEGF nor MSCs enhanced BMP-7 induced bone formation under the selected conditions. The present ceramic seemed to be osteoinductive and degradable, making this material suitable for bone tissue engineering. © 2010 European Association for Cranio-Maxillo-Facial Surgery.
APA:
Detsch, R. (2010). Bone formation and degradation of a highly porous biphasic calcium phosphate ceramic in presence of BMP-7, VEGF and mesenchymal stem cells in an ectopic mouse model. Journal of Cranio-Maxillofacial Surgery, 38(6), 423-430. https://doi.org/10.1016/j.jcms.2010.01.003
MLA:
Detsch, Rainer. "Bone formation and degradation of a highly porous biphasic calcium phosphate ceramic in presence of BMP-7, VEGF and mesenchymal stem cells in an ectopic mouse model." Journal of Cranio-Maxillofacial Surgery 38.6 (2010): 423-430.
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