Correlation between Supercoiling and Conformational Motions of the Bacterial Flagellar Filament

Beitrag in einer Fachzeitschrift


Details zur Publikation

Autorinnen und Autoren: Stadler AM, Unruh T, Namba K, Samatey F, Zaccai G
Zeitschrift: Biophysical Journal
Verlag: CELL PRESS
Jahr der Veröffentlichung: 2013
Band: 105
Heftnummer: 9
Seitenbereich: 2157-2165
ISSN: 0006-3495


Abstract


The bacterial flagellar filament is a very large macromolecular assembly of a single protein, flagellin. Various supercoiled states of the filament exist, which are formed by two structurally different conformations of flagellin in different ratios. We investigated the correlation between supercoiling of the protofilaments and molecular dynamics in the flagellar filament using quasielastic and elastic incoherent neutron scattering on the picosecond and nanosecond timescales. Thermal fluctuations in the straight L- and R-type filaments were measured and compared to the resting state of the wild-type filament. Amplitudes of motion on the picosecond timescale were found to be similar in the different conformational states. Mean-square displacements and protein resilience on the 0.1 ns timescale demonstrate that the L-type state is more flexible and less resilient than the R-type, whereas the wild-type state lies in between. Our results provide strong support that supercoiling of the protofilannents in the flagellar filament is determined by the strength of molecular forces in and between the flagellin subunits.



FAU-Autorinnen und Autoren / FAU-Herausgeberinnen und Herausgeber

Unruh, Tobias Prof. Dr.
Professur für Nanomaterialcharakterisierung (Streumethoden)


Einrichtungen weiterer Autorinnen und Autoren

Forschungszentrum Jülich GmbH (FZJ)
Institute Laue-Langevin (ILL)
Osaka University


Zitierweisen

APA:
Stadler, A.M., Unruh, T., Namba, K., Samatey, F., & Zaccai, G. (2013). Correlation between Supercoiling and Conformational Motions of the Bacterial Flagellar Filament. Biophysical Journal, 105(9), 2157-2165. https://dx.doi.org/10.1016/j.bpj.2013.09.039

MLA:
Stadler, Andreas M., et al. "Correlation between Supercoiling and Conformational Motions of the Bacterial Flagellar Filament." Biophysical Journal 105.9 (2013): 2157-2165.

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Zuletzt aktualisiert 2018-06-08 um 20:09