Functional characterisation and cell specificity of BvSUT1, the transporter that loads sucrose into the phloem of sugar beet (Beta vulgaris L.) source leaves.
Nieberl P, Ehrl C, Pommerrenig B, Graus D, Marten I, Jung B, Ludewig F, Koch W, Harms K, Flügge UI, Neuhaus HE, Hedrich R, Sauer N (2017)
Publication Language: English
Publication Status: Published
Publication Type: Journal article, Original article
Publication year: 2017
Journal
Book Volume: 19
Pages Range: 315-326
Journal Issue: 3
DOI: 10.1111/plb.12546
Open Access Link: http://onlinelibrary.wiley.com/doi/10.1111/plb.12546/abstract
Abstract
Sugar beet (Beta vulgaris L.) is one of the most important sugar-producing plants worldwide and provides about one third of the sugar consumed by humans. Here we report on molecular characterisation of the BvSUT1 gene and on the functional characterisation of the encoded transporter. In contrast to the recently identified tonoplast-localised sucrose transporter BvTST2.1 from sugar beet taproots, which evolved within the monosaccharide transporter (MST) superfamily, BvSUT1 represents a classical sucrose transporter and is a typical member of the disaccharide transporter (DST) superfamily. Transgenic Arabidopsis plants expressing the β-GLUCURONIDASE (GUS) reporter gene under control of the BvSUT1-promoter showed GUS histochemical staining of their phloem; an anti-BvSUT1-antiserum identified the BvSUT1 transporter specifically in phloem companion cells. After expression of BvSUT1 cDNA in bakers' yeasts (Saccharomyces cerevisiae) uptake characteristics of the BvSUT1 protein were studied. Moreover, the sugar beet transporter was characterised as a proton-coupled sucrose symporter in Xenopus laevis oocytes. Our findings indicate that BvSUT1 is the sucrose transporter that is responsible for loading of sucrose into the phloem of sugar beet source leaves delivering sucrose to the storage tissue in sugar beet taproot sinks.
Authors with CRIS profile
Christine Ehrl
Lehrstuhl für Molekulare Pflanzenphysiologie
Benjamin Pommerrenig
Lehrstuhl für Molekulare Pflanzenphysiologie
Norbert Sauer
Lehrstuhl für Molekulare Pflanzenphysiologie
Involved external institutions
Julius-Maximilians-Universität Würzburg
Germany (DE)
Technische Universität Kaiserslautern
Germany (DE)
Universität zu Köln
Germany (DE)
KWS SAAT SE
Germany (DE)
Südzucker AG
Germany (DE)
Germany (DE)
Technische Universität Kaiserslautern
Germany (DE)
Universität zu Köln
Germany (DE)
KWS SAAT SE
Germany (DE)
Südzucker AG
Germany (DE)
How to cite
APA:
Nieberl, P., Ehrl, C., Pommerrenig, B., Graus, D., Marten, I., Jung, B.,... Sauer, N. (2017). Functional characterisation and cell specificity of BvSUT1, the transporter that loads sucrose into the phloem of sugar beet (Beta vulgaris L.) source leaves. Plant Biology, 19(3), 315-326. https://dx.doi.org/10.1111/plb.12546
MLA:
Nieberl, Petra, et al. "Functional characterisation and cell specificity of BvSUT1, the transporter that loads sucrose into the phloem of sugar beet (Beta vulgaris L.) source leaves." Plant Biology 19.3 (2017): 315-326.
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