Performance of scientific cameras with different sensor types in measuring dynamic processes in fluorescence microscopy.

Jung J, Weisenburger S, Albert S, Gilbert D, Friedrich O, Eulenburg V, Kornhuber J, Groemer TW (2013)

Publication Status: Published

Publication Type: Journal article, Original article

Publication year: 2013

Journal

Microscopy research and technique

Book Volume: 76

Pages Range: 835-43

Journal Issue: 8

DOI: 10.1002/jemt.22236

Abstract

The plethora of available scientific cameras of different types challenges the biologically oriented experimenter when picking the appropriate camera for his experiment. In this study, we chose to investigate camera performances in a typical nonsingle molecule situation in life sciences, that is, quantitative measurements of fluorescence intensity changes from video data with typically skewed intensity distributions. Here, intensity profile dynamics of pH-sensors upon triggered changes of pH-environments in living cells served as a model system. The following camera types were tested: sCMOS, CCD (scientific and nonscientific) and EM-CCD (back- and front-illuminated). We found that although the EM-CCD cameras achieved the best absolute spatial SNR (signal-to-noise ratio) values, the sCMOS was at least of equal performance when the spatial SNR was related to the effective dynamic range, and it was superior in terms of temporal SNR. In the measurements of triggered intensity changes, the sCMOS camera had the advantage that it used the smallest fraction of its dynamic range when depicting intensity changes, and thus featured the best SNR at full usage of its dynamic range.

Authors with CRIS profile

Jasmin Jung Lehrstuhl für Psychiatrie und Psychotherapie Daniel Gilbert Lehrstuhl für Medizinische Biotechnologie Oliver Friedrich Lehrstuhl für Medizinische Biotechnologie Volker Eulenburg Lehrstuhl für Biochemie und Molekulare Medizin Johannes Kornhuber Lehrstuhl für Psychiatrie und Psychotherapie

Involved external institutions

Max-Planck-Institut für die Physik des Lichts (MPL) / Max Planck Institute for the Science of Light DE Germany (DE)

How to cite

APA:

Jung, J., Weisenburger, S., Albert, S., Gilbert, D., Friedrich, O., Eulenburg, V.,... Groemer, T.W. (2013). Performance of scientific cameras with different sensor types in measuring dynamic processes in fluorescence microscopy. Microscopy research and technique, 76(8), 835-43. https://doi.org/10.1002/jemt.22236

MLA:

Jung, Jasmin, et al. "Performance of scientific cameras with different sensor types in measuring dynamic processes in fluorescence microscopy." Microscopy research and technique 76.8 (2013): 835-43.

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