Motor protein function in skeletal abdominal muscle of cachectic cancer patients.

Taskin S, Stumpf VI, Bachmann J, Weber C, Martignoni ME, Friedrich O (2014)

Publication Language: English

Publication Status: Published

Publication Type: Journal article, Original article

Publication year: 2014

Journal

Journal of cellular and molecular medicine

Book Volume: 18

Pages Range: 69-79

Journal Issue: 1

URI: http://onlinelibrary.wiley.com/doi/10.1111/jcmm.12165/abstract;jsessionid=E5BDFC4245413F048ECA099352DF0B8B.f02t04

DOI: 10.1111/jcmm.12165

Open Access Link: http://onlinelibrary.wiley.com/doi/10.1111/jcmm.12165/epdf

Abstract

Cachexia presents with ongoing muscle wasting, altering quality of life in cancer patients. Cachexia is a limiting prognostic factor for patient survival and health care costs. Although animal models and human trials have shown mechanisms of motorprotein proteolysis, not much is known about intrinsic changes of muscle functionality in cancer patients suffering from muscle cachexia, and deeper insights into cachexia pathology in humans are needed. To address this question, rectus abdominis muscle samples were collected from several surgical control, non-cachectic and cachectic cancer patients and processed for skinned fibre biomechanics, molecular in vitro motility assays, myosin isoform protein compositions and quantitative ubiquitin polymer protein analysis. In pre-cachectic and cachectic cancer patient samples, maximum force was significantly compromised compared with controls, but showed an unexpected increase in myofibrillar Ca(2+) sensitivity consistent with a shift from slow to fast myosin isoform expression seen in SDS-PAGE analysis and in vitro motility assays. Force deficit was specific for 'cancer', but not linked to presence of cachexia. Interestingly, quantitative ubiquitin immunoassays revealed no major changes in static ubiquitin polymer protein profiles, whether cachexia was present or not and were shown to mirror profiles in control patients. Our study on muscle function in cachectic patients shows that abdominal wall skeletal muscle in cancer cachexia shows signs of weakness that can be partially attributed to intrinsic changes to contractile motorprotein function. On protein levels, static ubiquitin polymeric distributions were unaltered, pointing towards evenly up-regulated ubiquitin protein turnover with respect to ubiquitin conjugation, proteasome degradation and de-ubiquitination.

Authors with CRIS profile

Oliver Friedrich Lehrstuhl für Medizinische Biotechnologie

Related research project(s)

Critical Illness Myopathy: Motor protein function and contractility changes of skeletal muscle in muscle biopsies in critically ill ICU patients Oct. 1, 2009 - April 30, 2011

Involved external institutions

Ruprecht-Karls-Universität Heidelberg DE Germany (DE) Technische Universität München (TUM) DE Germany (DE)

How to cite

APA:

Taskin, S., Stumpf, V.I., Bachmann, J., Weber, C., Martignoni, M.E., & Friedrich, O. (2014). Motor protein function in skeletal abdominal muscle of cachectic cancer patients. Journal of cellular and molecular medicine, 18(1), 69-79. https://doi.org/10.1111/jcmm.12165

MLA:

Taskin, Sultan, et al. "Motor protein function in skeletal abdominal muscle of cachectic cancer patients." Journal of cellular and molecular medicine 18.1 (2014): 69-79.

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