Assessing viability of extracorporeal preserved muscle transplants using external field stimulation: a novel tool to improve methods prolonging bridge-to-transplantation time.

Taeger C, Friedrich O, Dragu A, Weigand A, Hobe F, Drechsler C, Geppert CI, Arkudas A, Münch F, Buchholz R, Pollmann C, Schramm A, Birkholz T, Horch RE, Präbst K, Buchholz R (2015)

Publication Language: English

Publication Status: Published

Publication Type: Journal article, Original article

Publication year: 2015

Journal

Scientific Reports Nature Publishing Group: Open Access Journals - Option B

Publisher: Nature Publishing Group: Open Access Journals - Option B

Book Volume: 5

Pages Range: 11956

DOI: 10.1038/srep11956

Abstract

Preventing ischemia-related cell damage is a priority when preserving tissue for transplantation. Perfusion protocols have been established for a variety of applications and proven to be superior to procedures used in clinical routine. Extracorporeal perfusion of muscle tissue though cumbersome is highly desirable since it is highly susceptible to ischemia-related damage. To show the efficacy of different perfusion protocols external field stimulation can be used to immediately visualize improvement or deterioration of the tissue during active and running perfusion protocols. This method has been used to show the superiority of extracorporeal perfusion using porcine rectus abdominis muscles perfused with heparinized saline solution. Perfused muscles showed statistically significant higher ability to exert force compared to nonperfused ones. These findings can be confirmed using Annexin V as marker for cell damage, perfusion of muscle tissue limits damage significantly compared to nonperfused tissue. The combination of extracorporeal perfusion and external field stimulation may improve organ conservation research.

Authors with CRIS profile

Christian Taeger Department of Plastic and Hand Surgery Oliver Friedrich Lehrstuhl für Medizinische Biotechnologie Annika Kengelbach-Weigand Department of Plastic and Hand Surgery Frieder Hobe Lehrstuhl für Bioverfahrenstechnik Carol-Immanuel Geppert Lehrstuhl für Allgemeine Pathologie und Pathologische Anatomie Andreas Arkudas Medizinische Fakultät Rainer Buchholz Technische Fakultät Charlotte Meyer Lehrstuhl für Medizinische Biotechnologie Torsten Birkholz Department of Anaesthesiology Raymund Horch Professur für Plastische Chirurgie und Handchirurgie Konstantin Präbst Lehrstuhl für Bioverfahrenstechnik

Involved external institutions

Klinikum St. Georg DE Germany (DE)

How to cite

APA:

Taeger, C., Friedrich, O., Dragu, A., Weigand, A., Hobe, F., Drechsler, C.,... Buchholz, R. (2015). Assessing viability of extracorporeal preserved muscle transplants using external field stimulation: a novel tool to improve methods prolonging bridge-to-transplantation time. Scientific Reports, 5, 11956. https://doi.org/10.1038/srep11956

MLA:

Taeger, Christian, et al. "Assessing viability of extracorporeal preserved muscle transplants using external field stimulation: a novel tool to improve methods prolonging bridge-to-transplantation time." Scientific Reports 5 (2015): 11956.

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