A portable low-cost long-term live-cell imaging platform for biomedical research and education.

Beitrag in einer Fachzeitschrift
(Originalarbeit)


Details zur Publikation

Autor(en): Walzik MP, Vollmar V, Lachnit T, Dietz H, Haug S, Bachmann H, Fath M, Aschenbrenner D, Abolpour Mofrad S, Friedrich O, Gilbert D
Zeitschrift: Biosensors and Bioelectronics
Jahr der Veröffentlichung: 2015
Band: 64
Seitenbereich: 639-49
ISSN: 0956-5663
Sprache: Englisch


Abstract


Time-resolved visualization and analysis of slow dynamic processes in living cells has revolutionized many aspects of in vitro cellular studies. However, existing technology applied to time-resolved live-cell microscopy is often immobile, costly and requires a high level of skill to use and maintain. These factors limit its utility to field research and educational purposes. The recent availability of rapid prototyping technology makes it possible to quickly and easily engineer purpose-built alternatives to conventional research infrastructure which are low-cost and user-friendly. In this paper we describe the prototype of a fully automated low-cost, portable live-cell imaging system for time-resolved label-free visualization of dynamic processes in living cells. The device is light-weight (3.6 kg), small (22 × 22 × 22 cm) and extremely low-cost (<€1250). We demonstrate its potential for biomedical use by long-term imaging of recombinant HEK293 cells at varying culture conditions and validate its ability to generate time-resolved data of high quality allowing for analysis of time-dependent processes in living cells. While this work focuses on long-term imaging of mammalian cells, the presented technology could also be adapted for use with other biological specimen and provides a general example of rapidly prototyped low-cost biosensor technology for application in life sciences and education.



FAU-Autoren / FAU-Herausgeber

Abolpour Mofrad, Sepideh
Lehrstuhl für Medizinische Biotechnologie
Friedrich, Oliver Prof. Dr. Dr.
Lehrstuhl für Medizinische Biotechnologie
Gilbert, Daniel PD Dr.
Lehrstuhl für Medizinische Biotechnologie


Zusätzliche Organisationseinheit(en)
Erlangen Graduate School in Advanced Optical Technologies


Zitierweisen

APA:
Walzik, M.P., Vollmar, V., Lachnit, T., Dietz, H., Haug, S., Bachmann, H.,... Gilbert, D. (2015). A portable low-cost long-term live-cell imaging platform for biomedical research and education. Biosensors and Bioelectronics, 64, 639-49. https://dx.doi.org/10.1016/j.bios.2014.09.061

MLA:
Walzik, Maria P., et al. "A portable low-cost long-term live-cell imaging platform for biomedical research and education." Biosensors and Bioelectronics 64 (2015): 639-49.

BibTeX: 

Zuletzt aktualisiert 2018-10-08 um 23:09