Impact of the nanoparticle-protein corona on colloidal stability and protein structure

Peukert W, Gebauer J, Malissek M, Simon S, Knauer S, Maskos M, Stauber R, Treuel L (2012)

Publication Language: English

Publication Status: Published

Publication Type: Journal article, Original article

Publication year: 2012

Journal

Langmuir American Chemical Society

Book Volume: 28

Pages Range: 9673-9679

Journal Issue: 25

DOI: 10.1021/la301104a

Abstract

In biological fluids, proteins may associate with nanoparticles (NPs), leading to the formation of a so-called "protein corona" largely defining the biological identity of the particle. Here, we present a novel approach to assess apparent binding affinities for the adsorption/desorption of proteins to silver NPs based on the impact of the corona formation on the agglomeration kinetics of the colloid. Affinities derived from circular dichroism measurements complement these results, simultaneously elucidating structural changes in the adsorbed protein. Employing human serum albumin as a model, apparent affinities in the nanomolar regime resulted from both approaches. Collectively, our findings now allow discrimination between the formation of protein mono- and multilayers on NP surfaces. © 2012 American Chemical Society.

Authors with CRIS profile

Wolfgang Peukert Lehrstuhl für Feststoff- und Grenzflächenverfahrenstechnik

Involved external institutions

Universität Duisburg-Essen (UDE) DE Germany (DE) Fachhochschule Mainz DE Germany (DE) Universitätsmedizin der Johannes Gutenberg-Universität Mainz DE Germany (DE) Karlsruhe Institute of Technology (KIT) DE Germany (DE)

How to cite

APA:

Peukert, W., Gebauer, J., Malissek, M., Simon, S., Knauer, S., Maskos, M.,... Treuel, L. (2012). Impact of the nanoparticle-protein corona on colloidal stability and protein structure. Langmuir, 28(25), 9673-9679. https://dx.doi.org/10.1021/la301104a

MLA:

Peukert, Wolfgang, et al. "Impact of the nanoparticle-protein corona on colloidal stability and protein structure." Langmuir 28.25 (2012): 9673-9679.

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