Phloem-specific expression of Yang cycle genes and identification of novel Yang cycle enzymes in Plantago and Arabidopsis.

Pommerrenig B, Feussner K, Zierer W, Rabinovych V, Klebl F, Feussner I, Sauer N (2011)

Publication Language: English

Publication Status: Published

Publication Type: Journal article, Original article

Publication year: 2011

Journal

The Plant Cell American Society of Plant Biologists

Book Volume: 23

Pages Range: 1904-1919

Journal Issue: 5

DOI: 10.1105/tpc.110.079657

Open Access Link: http://www.plantcell.org/content/23/5/1904.long

Abstract

The 5-methylthioadenosine (MTA) or Yang cycle is a set of reactions that recycle MTA to Met. In plants, MTA is a byproduct of polyamine, ethylene, and nicotianamine biosynthesis. Vascular transcriptome analyses revealed phloem-specific expression of the Yang cycle gene 5-METHYLTHIORIBOSE KINASE1 (MTK1) in Plantago major and Arabidopsis thaliana. As Arabidopsis has only a single MTK gene, we hypothesized that the expression of other Yang cycle genes might also be vascular specific. Reporter gene studies and quantitative analyses of mRNA levels for all Yang cycle genes confirmed this hypothesis for Arabidopsis and Plantago. This includes the Yang cycle genes 5-METHYLTHIORIBOSE-1-PHOSPHATE ISOMERASE1 and DEHYDRATASE-ENOLASE-PHOSPHATASE-COMPLEX1. We show that these two enzymes are sufficient for the conversion of methylthioribose-1-phosphate to 1,2-dihydroxy-3-keto-5-methylthiopentene. In bacteria, fungi, and animals, the same conversion is catalyzed in three to four separate enzymatic steps. Furthermore, comparative analyses of vascular and nonvascular metabolites identified Met, S-adenosyl Met, and MTA preferentially or almost exclusively in the vascular tissue. Our data represent a comprehensive characterization of the Yang cycle in higher plants and demonstrate that the Yang cycle works primarily in the vasculature. Finally, expression analyses of polyamine biosynthetic genes suggest that the Yang cycle in leaves recycles MTA derived primarily from polyamine biosynthesis.

Authors with CRIS profile

Benjamin Pommerrenig Lehrstuhl für Molekulare Pflanzenphysiologie Wolfgang Zierer Lehrstuhl für Molekulare Pflanzenphysiologie Franz Klebl Lehrstuhl für Molekulare Pflanzenphysiologie Norbert Sauer Lehrstuhl für Molekulare Pflanzenphysiologie

Involved external institutions

Georg-August-Universität Göttingen DE Germany (DE)

How to cite

APA:

Pommerrenig, B., Feussner, K., Zierer, W., Rabinovych, V., Klebl, F., Feussner, I., & Sauer, N. (2011). Phloem-specific expression of Yang cycle genes and identification of novel Yang cycle enzymes in Plantago and Arabidopsis. The Plant Cell, 23(5), 1904-1919. https://doi.org/10.1105/tpc.110.079657

MLA:

Pommerrenig, Benjamin, et al. "Phloem-specific expression of Yang cycle genes and identification of novel Yang cycle enzymes in Plantago and Arabidopsis." The Plant Cell 23.5 (2011): 1904-1919.

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