Induction of bone formation in biphasic calcium phosphate scaffolds by bone morphogenetic protein-2 and primary osteoblasts

Beitrag in einer Fachzeitschrift


Details zur Publikation

Autor(en): Strobel L, Rath SN, Maier AK, Beier J, Arkudas A, Greil P, Horch R, Kneser U
Zeitschrift: Journal of Tissue Engineering and Regenerative Medicine
Jahr der Veröffentlichung: 2014
Band: 8
Heftnummer: 3
Seitenbereich: 176-185
ISSN: 1932-6254


Abstract


Bone tissue engineering strategies mainly depend on porous scaffold materials. In this study, novel biphasic calcium phosphate (BCP) matrices were generated by 3D-printing. High porosity was achieved by starch consolidation. This study aimed to characterise the porous BCP-scaffold properties and interactions of osteogenic cells and growth factors under in vivo conditions. Five differently treated constructs were implanted subcutaneously in syngeneic rats: plain BCP constructs (group A), constructs pre-treated with BMP-2 (group B; 1.6 µg BMP-2 per scaffold), seeded with primary osteoblasts (OB) (group C), seeded with OB and BMP-2 (group D) and constructs seeded with OB and pre-cultivated in a flow bioreactor for 6 weeks (group E). After 2, 4 and 6 weeks, specimens were explanted and subjected to histological and molecular biological analyses. Explanted scaffolds were invaded by fibrovascular tissue without significant foreign body reactions. Morphometric analysis demonstrated significantly increased bone formation in samples from group D (OB + BMP-2) compared to all other groups. Samples from groups B-E displayed significant mRNA expression of bone-specific genes after 6 weeks. Pre-cultivation in the flow bioreactor (group E) induced bone formation comparable with group B. In this study, differences in bone distribution between samples with BMP-2 or osteoblasts could be observed. In conclusion, combination of osteoblasts and BMP-2 synergistically enhanced bone formation in novel ceramic scaffolds. These results provide the basis for further experiments in orthotopic defect models with a focus on future applications in orthopaedic and reconstructive surgery. Copyright © 2012 John Wiley & Sons, Ltd.



FAU-Autoren / FAU-Herausgeber

Arkudas, Andreas Prof. Dr.
Medizinische Fakultät
Greil, Peter Prof. Dr.
Lehrstuhl für Werkstoffwissenschaften (Glas und Keramik)
Horch, Raymund E. Prof. Dr.
Professur für Plastische Chirurgie und Handchirurgie
Maier, Anne-Kathrin
Lehrstuhl für Werkstoffwissenschaften (Glas und Keramik)
Strobel, Leonie
Professur für Plastische Chirurgie und Handchirurgie


Zitierweisen

APA:
Strobel, L., Rath, S.N., Maier, A.-K., Beier, J., Arkudas, A., Greil, P.,... Kneser, U. (2014). Induction of bone formation in biphasic calcium phosphate scaffolds by bone morphogenetic protein-2 and primary osteoblasts. Journal of Tissue Engineering and Regenerative Medicine, 8(3), 176-185. https://dx.doi.org/10.1002/term.1511

MLA:
Strobel, Leonie, et al. "Induction of bone formation in biphasic calcium phosphate scaffolds by bone morphogenetic protein-2 and primary osteoblasts." Journal of Tissue Engineering and Regenerative Medicine 8.3 (2014): 176-185.

BibTeX: 

Zuletzt aktualisiert 2018-05-10 um 02:31