Callose Biosynthesis Regulates Symplastic Trafficking during Root Development

Beitrag in einer Fachzeitschrift

Details zur Publikation

Autor(en): Vaten A, Dettmer J, Wu S, Stierhof YD, Miyashima S, Yadav SR, Roberts CJ, Campilho A, Bulone V, Lichtenberger R, Lehesranta S, Mahonen AP, Kim JY, Jokitalo E, Sauer N, Scheres B, Nakajima K, Carlsbecker A, Gallagher KL, Helariutta Y
Zeitschrift: Developmental Cell
Verlag: Elsevier (Cell Press)
Jahr der Veröffentlichung: 2011
Band: 21
Heftnummer: 6
Seitenbereich: 1144-1155
ISSN: 1534-5807
Sprache: Englisch


Plant cells are connected through plasmodesmata (PD), membrane-lined channels that allow symplastic movement of molecules between cells. However, little is known about the role of PD-mediated signaling during plant morphogenesis. Here, we describe an Arabidopsis gene, CALS3/GSL12. Gain-of-function mutations in CALS3 result in increased accumulation of callose (beta-1,3-glucan) at the PD, a decrease in PD aperture, defects in root development, and reduced intercellular trafficking. Enhancement of CALS3 expression during phloem development suppressed loss-of-function mutations in the phloem abundant callose synthase, CALS7 indicating that CALS3 is a bona fide callose synthase. CALS3 alleles allowed us to spatially and temporally control the PD aperture between plant tissues. Using this tool, we are able to show that movement of the transcription factor SHORT-ROOT and microRNA1 65 between the stele and the endodermis is PD dependent. Taken together, we conclude that regulated callose biosynthesis at PD is essential for cell signaling.

FAU-Autoren / FAU-Herausgeber

Dettmer, Jan Dr.
Lehrstuhl für Zellbiologie
Sauer, Norbert Prof. Dr.
Lehrstuhl für Molekulare Pflanzenphysiologie

Autor(en) der externen Einrichtung(en)
Eberhard Karls Universität Tübingen
Gyeongsang National University (GNU) / 경상대학교
Helsingin yliopisto / University of Helsinki
Nara Institute of Science and Technology (NAIST) / 奈良先端科学技術大学院大学
Royal Institute of Technology / Kungliga Tekniska Högskolan (KTH)
Universiteit Utrecht (UU) / Utrecht University
University of Pennsylvania
Uppsala University


Vaten, A., Dettmer, J., Wu, S., Stierhof, Y.-D., Miyashima, S., Yadav, S.R.,... Helariutta, Y. (2011). Callose Biosynthesis Regulates Symplastic Trafficking during Root Development. Developmental Cell, 21(6), 1144-1155.

Vaten, Anne, et al. "Callose Biosynthesis Regulates Symplastic Trafficking during Root Development." Developmental Cell 21.6 (2011): 1144-1155.


Zuletzt aktualisiert 2018-10-08 um 11:09