Reflective properties of the stigma in male gametes of Ectocarpus siliculosus (Phaeophyceae) studied by confocal laser scanning microscopy

Journal article


Publication Details

Author(s): Kreimer G, Kawai H, Müller DG, Melkonian M
Journal: Journal of Phycology
Publisher: Wiley-Blackwell
Publication year: 1991
Volume: 27
Journal issue: 2
Pages range: 268-276
ISSN: 0022-3646


Abstract


The reflection properties of the stigma in male gametes of Ectocarpus siliculosus (Dillw.) Lyngbye were investigated using confocal laser scanning microscopy in the epireflection contrast mode. The complex reflection pattern obtained after optical xy (horizontal) and xz (vertical) sectioning was consistent with stigma ultrastructure as revealed by serial thin sections. The intensity and pattern of the reflection signal varied with the orientation of the cell/stigma to the incident laser light. Maximal reflection occurred only in approximately normal orientation of the stigma to the light source. Focusing of reflected light from an elongated concave depression of the stigma on the region of the flagellar swelling was observed in xy and xz sections of living and fixed gametes. The results indicate the importance of mechanisms (focusing) other than quarter-wave interference reflection in signal amplification by the eyespot of flagellate algae.




External institutions
Hokkaido University (Hokudai) / 北海道大学
Universität Konstanz
Universität zu Köln


How to cite

APA:
Kreimer, G., Kawai, H., Müller, D.G., & Melkonian, M. (1991). Reflective properties of the stigma in male gametes of Ectocarpus siliculosus (Phaeophyceae) studied by confocal laser scanning microscopy. Journal of Phycology, 27(2), 268-276. https://dx.doi.org/10.1111/j.0022-3646.1991.00268.x

MLA:
Kreimer, Georg, et al. "Reflective properties of the stigma in male gametes of Ectocarpus siliculosus (Phaeophyceae) studied by confocal laser scanning microscopy." Journal of Phycology 27.2 (1991): 268-276.

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Last updated on 2018-11-08 at 02:27