The Heme-Binding Protein SOUL3 of Chlamydomonas reinhardtii Influences Size and Position of the Eyespot

Journal article


Publication Details

Author(s): Schulze T, Schreiber S, Iliev D, Boesger J, Trippens J, Kreimer G, Mittag M
Journal: Molecular Plant
Publisher: Oxford University Press (OUP): Policy B - Oxford Open Option D
Publication year: 2013
Volume: 6
Journal issue: 3
Pages range: 931-944
ISSN: 1674-2052


Abstract


The heme-binding protein SOUL3 of Chlamydomonas reinhardtii is primarily localized in the pigment globule layer of the eyespot, a primitive visual system. SOUL3 is involved in determining the size and position of the eyespot.The flagellated green alga Chlamydomonas reinhardtii has a primitive visual system, the eyespot. It is situated at the cells equator and allows the cell to phototax. In a previous proteomic analysis of the eyespot, the SOUL3 protein was identified among 202 proteins. Here, we investigate the properties and functions of SOUL3. Heterologously expressed SOUL3 is able to bind specifically to hemin. In C. reinhardtii, SOUL3 is expressed at a constant level over the diurnal cycle, but forms protein complexes that differ in size during day and night phases. SOUL3 is primarily localized in the eyespot and it is situated in the pigment globule layer thereof. This is in contrast to the channelrhodopsin photoreceptors, which are localized in the plasma membrane region of the eyespot. Knockdown lines with a significantly reduced SOUL3 level are characterized by mislocalized eyespots, a decreased eyespot size, and alterations in phototactic behavior. Mislocalizations were either anterior or posterior and did not affect association with acetylated microtubules of the daughter four-membered rootlet. Our data suggest that SOUL3 is involved in the organization and placement of the eyespot within the cell.



FAU Authors / FAU Editors

Kreimer, Georg Prof. Dr.
Professur für Zellbiologie
Trippens, Jessica Dr. rer. nat.
Professur für Zellbiologie


External institutions
Friedrich-Schiller-Universität Jena


How to cite

APA:
Schulze, T., Schreiber, S., Iliev, D., Boesger, J., Trippens, J., Kreimer, G., & Mittag, M. (2013). The Heme-Binding Protein SOUL3 of Chlamydomonas reinhardtii Influences Size and Position of the Eyespot. Molecular Plant, 6(3), 931-944. https://dx.doi.org/10.1093/mp/sss137

MLA:
Schulze, Thomas, et al. "The Heme-Binding Protein SOUL3 of Chlamydomonas reinhardtii Influences Size and Position of the Eyespot." Molecular Plant 6.3 (2013): 931-944.

BibTeX: 

Last updated on 2018-06-08 at 14:54