The iBeetle large-scale RNAi screen reveals gene functions for insect development and physiology

Beitrag in einer Fachzeitschrift

Details zur Publikation

Autorinnen und Autoren: Schmitt-Engel C, Schultheis D, Schwirz J, Ströhlein N, Troelenberg N, Majumdar U, Dao VA, Grossmann D, Richter T, Tech M, Doenitz J, Gerischer L, Theis M, Schild I, Trauner J, Koniszewski NDB, Kuester E, Kittelmann S, Hu Y, Lehmann S, Siemanowski J, Ulrich J, Panfilio KA, Schroeder R, Morgenstern B, Stanke M, Buchhholz F, Frasch M, Roth S, Wimmer EA, Schoppmeier M, Klingler M, Bucher G
Zeitschrift: Nature Communications
Verlag: Nature Publishing Group: Nature Communications
Jahr der Veröffentlichung: 2015
Band: 6
ISSN: 2041-1723


Genetic screens are powerful tools to identify the genes required for a given biological process. However, for technical reasons, comprehensive screens have been restricted to very few model organisms. Therefore, although deep sequencing is revealing the genes of ever more insect species, the functional studies predominantly focus on candidate genes previously identified in Drosophila, which is biasing research towards conserved gene functions. RNAi screens in other organisms promise to reduce this bias. Here we present the results of the iBeetle screen, a large-scale, unbiased RNAi screen in the red flour beetle, Tribolium castaneum, which identifies gene functions in embryonic and postembryonic development, physiology and cell biology. The utility of Tribolium as a screening platform is demonstrated by the identification of genes involved in insect epithelial adhesion. This work transcends the restrictions of the candidate gene approach and opens fields of research not accessible in Drosophila.

FAU-Autorinnen und Autoren / FAU-Herausgeberinnen und Herausgeber

Frasch, Manfred Prof. Dr.
Lehrstuhl für Entwicklungsbiologie
Klingler, Martin Prof. Dr.
Professur für Zoologie
Majumdar, Upalparna
Lehrstuhl für Entwicklungsbiologie
Richter, Tobias
Lehrstuhl für Entwicklungsbiologie
Schoppmeier, Michael PD Dr.
Lehrstuhl für Entwicklungsbiologie
Schultheis, Dorothea
Lehrstuhl für Entwicklungsbiologie
Ströhlein, Nadi
Lehrstuhl für Entwicklungsbiologie
Trauner, Jochen Dr.
Lehrstuhl für Entwicklungsbiologie
Troelenberg, Nicole
Professur für Zoologie

Einrichtungen weiterer Autorinnen und Autoren

Georg-August-Universität Göttingen
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Schmitt-Engel, C., Schultheis, D., Schwirz, J., Ströhlein, N., Troelenberg, N., Majumdar, U.,... Bucher, G. (2015). The iBeetle large-scale RNAi screen reveals gene functions for insect development and physiology. Nature Communications, 6.

Schmitt-Engel, Christian, et al. "The iBeetle large-scale RNAi screen reveals gene functions for insect development and physiology." Nature Communications 6 (2015).


Zuletzt aktualisiert 2018-24-11 um 13:50