Ultra high frequency ultrasound enables real-time visualization of blood supply from chorioallantoic membrane to human autosomal dominant polycystic kidney tissue
Schueler J, Kuenzel J, Thuesing A, Pion E, Behncke RY, Haegerling R, Fuchs D, Kraus A, Buchholz B, Huang B, Merhof D, Werner JM, Schmidt KM, Hackl C, Aung T, Haerteis S (2024)
Publication Type: Journal article
Publication year: 2024
Journal
Book Volume: 14
Article Number: 10063
Journal Issue: 1
DOI: 10.1038/s41598-024-60783-3
Abstract
Ultra high frequency (UHF) ultrasound enables the visualization of very small structures that cannot be detected by conventional ultrasound. The utilization of UHF imaging as a new imaging technique for the 3D-in-vivo chorioallantoic membrane (CAM) model can facilitate new insights into tissue perfusion and survival. Therefore, human renal cystic tissue was grafted onto the CAM and examined using UHF ultrasound imaging. Due to the unprecedented resolution of UHF ultrasound, it was possible to visualize microvessels, their development, and the formation of anastomoses. This enabled the observation of anastomoses between human and chicken vessels only 12 h after transplantation. These observations were validated by 3D reconstructions from a light sheet microscopy image stack, indocyanine green angiography, and histological analysis. Contrary to the assumption that the nutrient supply of the human cystic tissue and the gas exchange happens through diffusion from CAM vessels, this study shows that the vasculature of the human cystic tissue is directly connected to the blood vessels of the CAM and perfusion is established within a short period. Therefore, this in-vivo model combined with UHF imaging appears to be the ideal platform for studying the effects of intravenously applied therapeutics to inhibit renal cyst growth.
Authors with CRIS profile
Andre Kraus
Department of Medicine 4 – Nephrology and Hypertension
Björn Buchholz
Medizinische Fakultät
Involved external institutions
Charité - Universitätsmedizin Berlin
Germany (DE)
Universität Regensburg
Germany (DE)
Universitätsklinikum Regensburg
Germany (DE)
Germany (DE)
Universität Regensburg
Germany (DE)
Universitätsklinikum Regensburg
Germany (DE)
How to cite
APA:
Schueler, J., Kuenzel, J., Thuesing, A., Pion, E., Behncke, R.Y., Haegerling, R.,... Haerteis, S. (2024). Ultra high frequency ultrasound enables real-time visualization of blood supply from chorioallantoic membrane to human autosomal dominant polycystic kidney tissue. Scientific Reports, 14(1). https://doi.org/10.1038/s41598-024-60783-3
MLA:
Schueler, Jan, et al. "Ultra high frequency ultrasound enables real-time visualization of blood supply from chorioallantoic membrane to human autosomal dominant polycystic kidney tissue." Scientific Reports 14.1 (2024).
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