A vascularized in vivo melanoma model suitable for metastasis research of different tumor stages using fundamentally different bioinks

Schmid R, Schmidt S, Schrüfer S, Schubert DW, Heltmann-Meyer S, Schicht M, Paulsen F, Horch RE, Boßerhoff AK, Kengelbach-Weigand A, Arkudas A (2024)

Publication Type: Journal article

Publication year: 2024

Journal

Materials Today Bio Elsevier

Book Volume: 26

Pages Range: 101071

Article Number: 101071

DOI: 10.1016/j.mtbio.2024.101071

Abstract

Although 2D cancer models have been the standard for drug development, they don't resemble in vivo properties adequately. 3D models can potentially overcome this. Bioprinting is a promising technique for more refined models to investigate central processes in tumor development such as proliferation, dormancy or metastasis.


We aimed to analyze bioinks, which could mimic these different tumor stages in a cast vascularized arteriovenous loop melanoma model in vivo. It has the advantage to be a closed system with a defined microenvironment, supplied only with one vessel—ideal for metastasis research.


Tested bioinks showed significant differences in composition, printability, stiffness and microscopic pore structure, which led to different tumor stages (Matrigel and Alg/HA/Gel for progression, Cellink Bioink for dormancy) and resulted in different primary tumor growth (Matrigel significantly higher than Cellink Bioink). Light-sheet fluorescence microscopy revealed differences in vascularization and hemorrhages with no additional vessels found in Cellink Bioink. Histologically, typical human melanoma with different stages was demonstrated. HMB-45-positive tumors in progression inks were infiltrated by macrophages (CD163), highly proliferative (Ki67) and metastatic (MITF/BRN2, ATX, MMP3). Stainings of lymph nodes revealed metastases even without significant primary tumor growth in Cellink Bioink.


This model can be used to study tumor pathology and metastasis of different tumor stages and therapies

Authors with CRIS profile

Rafael Schmid Department of Plastic and Hand Surgery Sonja Schmidt Lehrstuhl für Biochemie und Molekulare Medizin Stefan Schrüfer Lehrstuhl für Werkstoffwissenschaften (Polymerwerkstoffe) Dirk Schubert Lehrstuhl für Werkstoffwissenschaften (Polymerwerkstoffe) Stefanie Heltmann-Meyer Department of Plastic and Hand Surgery Martin Schicht Lehrstuhl für Funktionelle und Klinische Anatomie Friedrich Paulsen Lehrstuhl für Funktionelle und Klinische Anatomie Raymund Horch Professur für Plastische Chirurgie und Handchirurgie Anja Katrin Boßerhoff Lehrstuhl für Biochemie und Molekulare Medizin Annika Kengelbach-Weigand Department of Plastic and Hand Surgery Andreas Arkudas Professur für Plastische Chirurgie und Handchirurgie

Additional Organisation(s)

Lehrstuhl für Werkstoffwissenschaften (Polymerwerkstoffe)

How to cite

APA:

Schmid, R., Schmidt, S., Schrüfer, S., Schubert, D.W., Heltmann-Meyer, S., Schicht, M.,... Arkudas, A. (2024). A vascularized in vivo melanoma model suitable for metastasis research of different tumor stages using fundamentally different bioinks. Materials Today Bio, 26, 101071. https://doi.org/10.1016/j.mtbio.2024.101071

MLA:

Schmid, Rafael, et al. "A vascularized in vivo melanoma model suitable for metastasis research of different tumor stages using fundamentally different bioinks." Materials Today Bio 26 (2024): 101071.

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