A paintbrush for delivery of nanoparticles and molecules to live cells with precise spatiotemporal control

Holler C, Taylor RW, Schambony A, Möckl L, Sandoghdar V (2024)

Publication Type: Journal article

Publication year: 2024

Journal

Nature methods

DOI: 10.1038/s41592-024-02177-x

Abstract

Delivery of very small amounts of reagents to the near-field of cells with micrometer spatial precision and millisecond time resolution is currently out of reach. Here we present μkiss as a micropipette-based scheme for brushing a layer of small molecules and nanoparticles onto the live cell membrane from a subfemtoliter confined volume of a perfusion flow. We characterize our system through both experiments and modeling, and find excellent agreement. We demonstrate several applications that benefit from a controlled brush delivery, such as a direct means to quantify local and long-range membrane mobility and organization as well as dynamical probing of intercellular force signaling.

Authors with CRIS profile

Vahid Sandoghdar

Additional Organisation(s)

FAU Profilzentrum Licht.Materie.Quantentechnologien (FAU LMQ)

Involved external institutions

Max-Planck-Institut für die Physik des Lichts (MPL) / Max Planck Institute for the Science of Light DE Germany (DE)

How to cite

APA:

Holler, C., Taylor, R.W., Schambony, A., Möckl, L., & Sandoghdar, V. (2024). A paintbrush for delivery of nanoparticles and molecules to live cells with precise spatiotemporal control. Nature methods. https://doi.org/10.1038/s41592-024-02177-x

MLA:

Holler, Cornelia, et al. "A paintbrush for delivery of nanoparticles and molecules to live cells with precise spatiotemporal control." Nature methods (2024).

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