Cytoskeletal coherence requires myosin-IIA contractility
Cai Y, Rossier O, Gauthier NC, Biais N, Fardin MA, Zhang X, Miller LW, Ladoux B, Cornish VW, Sheetz MP (2010)
Publication Type: Journal article
Publication year: 2010
Journal
Book Volume: 123
Pages Range: 413-423
Journal Issue: 3
DOI: 10.1242/jcs.058297
Abstract
Maintaining a physical connection across cytoplasm is crucial for many biological processes such as matrix force generation, cell motility, cell shape and tissue development. However, in the absence of stress fibers, the coherent structure that transmits force across the cytoplasm is not understood. We find that nonmuscle myosin-II (NMII) contraction of cytoplasmic actin filaments establishes a coherent cytoskeletal network irrespective of the nature of adhesive contacts. When NMII activity is inhibited during cell spreading by Rho kinase inhibition, blebbistatin, caldesmon overexpression or NMIIA RNAi, the symmetric traction forces are lost and cell spreading persists, causing cytoplasm fragmentation by membrane tension that results in 'C' or dendritic shapes. Moreover, local inactivation of NMII by chromophore-assisted laser inactivation causes local loss of coherence. Actin filament polymerization is also required for cytoplasmic coherence, but microtubules and intermediate filaments are dispensable. Loss of cytoplasmic coherence is accompanied by loss of circumferential actin bundles. We suggest that NMIIA creates a coherent actin network through the formation of circumferential actin bundles that mechanically link elements of the peripheral actin cytoskeleton where much of the force is generated during spreading.
Authors with CRIS profile
Involved external institutions
Columbia University
United States (USA) (US)
University of Paris 7 - Denis Diderot / Université Paris VII Denis Diderot
France (FR)
University of Illinois at Chicago
United States (USA) (US)
United States (USA) (US)
University of Paris 7 - Denis Diderot / Université Paris VII Denis Diderot
France (FR)
University of Illinois at Chicago
United States (USA) (US)
How to cite
APA:
Cai, Y., Rossier, O., Gauthier, N.C., Biais, N., Fardin, M.A., Zhang, X.,... Sheetz, M.P. (2010). Cytoskeletal coherence requires myosin-IIA contractility. Journal of Cell Science, 123(3), 413-423. https://doi.org/10.1242/jcs.058297
MLA:
Cai, Yunfei, et al. "Cytoskeletal coherence requires myosin-IIA contractility." Journal of Cell Science 123.3 (2010): 413-423.
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