Adhesion-mediated heterogeneous actin organization governs apoptotic cell extrusion
Le AP, Rupprecht JF, Mège RM, Toyama Y, Lim CT, Ladoux B (2021)
Publication Type: Journal article
Publication year: 2021
Journal
Book Volume: 12
Article Number: 397
Journal Issue: 1
DOI: 10.1038/s41467-020-20563-9
Abstract
Apoptotic extrusion is crucial in maintaining epithelial homeostasis. Current literature supports that epithelia respond to extrusion by forming a supracellular actomyosin purse-string in the neighbors. However, whether other actin structures could contribute to extrusion and how forces generated by these structures can be integrated are unknown. Here, we found that during extrusion, a heterogeneous actin network composed of lamellipodia protrusions and discontinuous actomyosin cables, was reorganized in the neighboring cells. The early presence of basal lamellipodia protrusion participated in both basal sealing of the extrusion site and orienting the actomyosin purse-string. The co-existence of these two mechanisms is determined by the interplay between the cell-cell and cell-substrate adhesions. A theoretical model integrates these cellular mechanosensitive components to explain why a dual-mode mechanism, which combines lamellipodia protrusion and purse-string contractility, leads to more efficient extrusion than a single-mode mechanism. In this work, we provide mechanistic insight into extrusion, an essential epithelial homeostasis process.
Authors with CRIS profile
Involved external institutions
National University of Singapore (NUS)
Singapore (SG)
Université Paris-Sud
France (FR)
National Center for Scientific Research / Centre national de la recherche scientifique (CNRS)
France (FR)
Singapore (SG)
Université Paris-Sud
France (FR)
National Center for Scientific Research / Centre national de la recherche scientifique (CNRS)
France (FR)
How to cite
APA:
Le, A.P., Rupprecht, J.F., Mège, R.M., Toyama, Y., Lim, C.T., & Ladoux, B. (2021). Adhesion-mediated heterogeneous actin organization governs apoptotic cell extrusion. Nature Communications, 12(1). https://doi.org/10.1038/s41467-020-20563-9
MLA:
Le, Anh Phuong, et al. "Adhesion-mediated heterogeneous actin organization governs apoptotic cell extrusion." Nature Communications 12.1 (2021).
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