Myenteric Plexus Immune Cell Infiltrations and Neurotransmitter Expression in Crohn’s Disease and Ulcerative Colitis

Wiese JJ, Manna S, Kühl AA, Fascì A, Elezkurtaj S, Sonnenberg E, Bubeck M, Atreya R, Becker C, Weixler B, Siegmund B, Patankar J, Prüß MS, Schumann M (2024)

Publication Type: Journal article

Publication year: 2024

Journal

Book Volume: 18

Pages Range: 121-133

Journal Issue: 1

DOI: 10.1093/ecco-jcc/jjad122

Abstract

Background and Aims: Pain is a cardinal symptom in inflammatory bowel disease [IBD]. An important structure in the transduction of pain signalling is the myenteric plexus [MP]. Nevertheless, IBD-associated infiltration of the MP by immune cells lacks in-depth characterisation. Herein, we decipher intra- and periganglionic immune cell infiltrations in Crohn´s disease [CD] and ulcerative colitis [UC] and provide a comparison with murine models of colitis. Methods: Full wall specimens of surgical colon resections served to examine immune cell populations by either conventional immunohistochemistry or immunofluorescence followed by either bright field or confocal microscopy. Results were compared with equivalent examinations in various murine models of intestinal inflammation. Results: Whereas the MP morphology was not significantly altered in IBD, we identified intraganglionic IBD-specific B cell- and monocyte-dominant cell infiltrations in CD. In contrast, UC-MPs were infiltrated by CD8⁺ T cells and revealed a higher extent of ganglionic cell apoptosis. With regard to the murine models of intestinal inflammation, the chronic dextran sulphate sodium [DSS]-induced colitis model reflected CD [and to a lesser extent UC] best, as it also showed increased monocytic infiltration as well as a modest B cell and CD8⁺ T cell infiltration. Conclusions: In CD, MPs were infiltrated by B cells and monocytes. In UC, mostly CD8⁺ cytotoxic T cells were found. The chronic DSS-induced colitis in the mouse model reflected best the MP-immune cell infiltrations representative for IBD.

Authors with CRIS profile

Involved external institutions

Charité - Universitätsmedizin Berlin Germany (DE) Berliner Institut für Gesundheitsforschung (BIH) Germany (DE)

How to cite

APA:

Wiese, J.J., Manna, S., Kühl, A.A., Fascì, A., Elezkurtaj, S., Sonnenberg, E.,... Schumann, M. (2024). Myenteric Plexus Immune Cell Infiltrations and Neurotransmitter Expression in Crohn’s Disease and Ulcerative Colitis. Journal of Crohns & Colitis, 18(1), 121-133. https://doi.org/10.1093/ecco-jcc/jjad122

MLA:

Wiese, Jakob J., et al. "Myenteric Plexus Immune Cell Infiltrations and Neurotransmitter Expression in Crohn’s Disease and Ulcerative Colitis." Journal of Crohns & Colitis 18.1 (2024): 121-133.

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