Programming of neural progenitors of the adult subependymal zone towards a glutamatergic neuron lineage by neurogenin 2

Péron S, Miyakoshi LM, Brill MS, Manzano-Franco D, Serrano-López J, Fan W, Marichal N, Ghanem A, Conzelmann KK, Karow M, Ortega F, Gascón S, Berninger B (2023)

Publication Type: Journal article

Publication year: 2023

Journal

Stem Cell Reports Elsevier

Book Volume: 18

Pages Range: 2418-2433

Journal Issue: 12

DOI: 10.1016/j.stemcr.2023.10.019

Abstract

Although adult subependymal zone (SEZ) neural stem cells mostly generate GABAergic interneurons, a small progenitor population expresses the proneural gene Neurog2 and produces glutamatergic neurons. Here, we determined whether Neurog2 could respecify SEZ neural stem cells and their progeny toward a glutamatergic fate. Retrovirus-mediated expression of Neurog2 induced the glutamatergic lineage markers TBR2 and TBR1 in cultured SEZ progenitors, which differentiated into functional glutamatergic neurons. Likewise, Neurog2-transduced SEZ progenitors acquired glutamatergic neuron hallmarks in vivo. Intriguingly, they failed to migrate toward the olfactory bulb and instead differentiated within the SEZ or the adjacent striatum, where they received connections from local neurons, as indicated by rabies virus-mediated monosynaptic tracing. In contrast, lentivirus-mediated expression of Neurog2 failed to reprogram early SEZ neurons, which maintained GABAergic identity and migrated to the olfactory bulb. Our data show that NEUROG2 can program SEZ progenitors toward a glutamatergic identity but fails to reprogram their neuronal progeny.

Authors with CRIS profile

Marisa Karow-Falk Professur für Biochemie und Molekulare Neurowissenschaften

Involved external institutions

Universitätsmedizin der Johannes Gutenberg-Universität Mainz DE Germany (DE) Ludwig-Maximilians-Universität (LMU) DE Germany (DE) Universidad Complutense de Madrid (UCM) ES Spain (ES) Technische Universität München (TUM) DE Germany (DE) Instituto de Neurobiología Ramón y Cajal ES Spain (ES) King’s College London GB United Kingdom (GB)

How to cite

APA:

Péron, S., Miyakoshi, L.M., Brill, M.S., Manzano-Franco, D., Serrano-López, J., Fan, W.,... Berninger, B. (2023). Programming of neural progenitors of the adult subependymal zone towards a glutamatergic neuron lineage by neurogenin 2. Stem Cell Reports, 18(12), 2418-2433. https://doi.org/10.1016/j.stemcr.2023.10.019

MLA:

Péron, Sophie, et al. "Programming of neural progenitors of the adult subependymal zone towards a glutamatergic neuron lineage by neurogenin 2." Stem Cell Reports 18.12 (2023): 2418-2433.

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