Re-Analysis of the Widely Used Recombinant Murine Cytomegalovirus MCMV-m157luc Derived from the Bacmid pSM3fr Confirms Its Hybrid Nature

Cordsmeier A, Bednar C, Kübel S, Bauer L, Enßer A (2023)

Publication Type: Journal article

Publication year: 2023

Journal

International Journal of Molecular Sciences MDPI

Book Volume: 24

Article Number: 14102

Journal Issue: 18

DOI: 10.3390/ijms241814102

Abstract

Murine cytomegalovirus (MCMV), and, in particular, recombinant virus derived from MCMV-bacmid pSM3fr, is widely used as the small animal infection model for human cytomegalovirus (HCMV). We sequenced the complete genomes of MCMV strains and recombinants for quality control. However, we noticed deviances from the deposited reference sequences of MCMV-bacmid pSM3fr. This prompted us to re-analyze pSM3fr and reannotate the reference sequence, as well as that for the commonly used MCMV-m157luc reporter virus. A correct reference sequence for this frequently used pSM3fr, containing a repaired version of m129 (MCK-2) and the luciferase gene instead of ORF m157, was constructed. The new reference also contains the original bacmid sequence, and it has a hybrid origin from MCMV strains Smith and K181.

Authors with CRIS profile

Arne Cordsmeier Institute of Clinical and Molecular Virology Christopher Bednar Institute of Clinical and Molecular Virology Sabrina Kübel Graduiertenzentrum der FAU Larissa Bauer Institute of Clinical and Molecular Virology Armin Enßer Institute of Clinical and Molecular Virology

How to cite

APA:

Cordsmeier, A., Bednar, C., Kübel, S., Bauer, L., & Enßer, A. (2023). Re-Analysis of the Widely Used Recombinant Murine Cytomegalovirus MCMV-m157luc Derived from the Bacmid pSM3fr Confirms Its Hybrid Nature. International Journal of Molecular Sciences, 24(18). https://doi.org/10.3390/ijms241814102

MLA:

Cordsmeier, Arne, et al. "Re-Analysis of the Widely Used Recombinant Murine Cytomegalovirus MCMV-m157luc Derived from the Bacmid pSM3fr Confirms Its Hybrid Nature." International Journal of Molecular Sciences 24.18 (2023).

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