Isolation and profiling of viable tumor cells from human ex vivo glioblastoma cultures through single-cell transcriptomics

Zhang J, Straehle J, Joseph K, Neidert N, Behringer S, Göldner J, Vlachos A, Prinz M, Fung C, Beck J, Schnell O, Heiland DH, Ravi VM (2023)

Publication Type: Journal article

Publication year: 2023

Journal

STAR Protocols Cell Press

Book Volume: 4

Article Number: 102383

Journal Issue: 3

DOI: 10.1016/j.xpro.2023.102383

Abstract

Single-cell RNA-sequencing (scRNA-seq) is becoming a ubiquitous method in profiling the cellular transcriptomes of both malignant and non-malignant cells from the human brain. Here, we present a protocol to isolate viable tumor cells from human ex vivo glioblastoma cultures for single-cell transcriptomic analysis. We describe steps including surgical tissue collection, sectioning, culturing, primary tumor cells inoculation, growth tracking, fluorescence-based cell sorting, and population-enriched scRNA-seq. This comprehensive methodology empowers in-depth understanding of brain tumor biology at the single-cell level. For complete details on the use and execution of this protocol, please refer to Ravi et al.1

Authors with CRIS profile

Oliver Schnell

Involved external institutions

Universitätsklinikum Freiburg DE Germany (DE) Albert-Ludwigs-Universität Freiburg DE Germany (DE)

How to cite

APA:

Zhang, J., Straehle, J., Joseph, K., Neidert, N., Behringer, S., Göldner, J.,... Ravi, V.M. (2023). Isolation and profiling of viable tumor cells from human ex vivo glioblastoma cultures through single-cell transcriptomics. STAR Protocols, 4(3). https://doi.org/10.1016/j.xpro.2023.102383

MLA:

Zhang, Junyi, et al. "Isolation and profiling of viable tumor cells from human ex vivo glioblastoma cultures through single-cell transcriptomics." STAR Protocols 4.3 (2023).

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