Label-free discrimination of extracellular vesicles from large lipoproteins.
Kashkanova AD, Blessing M, Reischke M, Baur JO, Baur A, Sandoghdar V, Van Deun J (2023)
Publication Type: Journal article
Publication year: 2023
Journal
Book Volume: 12
Journal Issue: 8
DOI: 10.1002/jev2.12348
Abstract
Extracellular vesicles (EVs) are increasingly gaining interest as biomarkers and therapeutics. Accurate sizing and quantification of EVs remain problematic, given their nanometre size range and small scattering cross-sections. This is compounded by the fact that common EV isolation methods result in co-isolation of particles with comparable features. Especially in blood plasma, similarly-sized lipoproteins outnumber EVs to a great extent. Recently, interferometric nanoparticle tracking analysis (iNTA) was introduced as a particle analysis method that enables determining the size and refractive index of nanoparticles with high sensitivity and precision. In this work, we apply iNTA to differentiate between EVs and lipoproteins, and compare its performance to conventional nanoparticle tracking analysis (NTA). We show that iNTA can accurately quantify EVs in artificial EV-lipoprotein mixtures and in plasma-derived EV samples of varying complexity. Conventional NTA could not report on EV numbers, as it was not able to distinguish EVs from lipoproteins. iNTA has the potential to become a new standard for label-free EV characterization in suspension.
Authors with CRIS profile
Jan-Ole Baur
Department of Dermatology
Andreas Baur
Department of Dermatology
Vahid Sandoghdar
Jan Van Deun Department of Dermatology
Jan Van Deun Department of Dermatology
Additional Organisation(s)
Involved external institutions
Germany (DE)How to cite
APA:
Kashkanova, A.D., Blessing, M., Reischke, M., Baur, J.-O., Baur, A., Sandoghdar, V., & Van Deun, J. (2023). Label-free discrimination of extracellular vesicles from large lipoproteins. Journal of Extracellular Vesicles, 12(8). https://doi.org/10.1002/jev2.12348
MLA:
Kashkanova, Anna D., et al. "Label-free discrimination of extracellular vesicles from large lipoproteins." Journal of Extracellular Vesicles 12.8 (2023).
BibTeX: Download