Label-Free Molecular Imaging of Biological Cells and Tissues by Linear and Nonlinear Raman Spectroscopic Approaches

Krafft C, Schmitt M, Schie IW, Cialla-May D, Matthaeus C, Bocklitz T, Popp J (2017)

Publication Type: Journal article, Review article

Publication year: 2017

Journal

Angewandte Chemie International Edition Wiley

Book Volume: 56

Pages Range: 4392-4430

Journal Issue: 16

DOI: 10.1002/anie.201607604

Abstract

Raman spectroscopy is an emerging technique in bioanalysis and imaging of biomaterials owing to its unique capability of generating spectroscopic fingerprints. Imaging cells and tissues by Raman microspectroscopy represents a nondestructive and label-free approach. All components of cells or tissues contribute to the Raman signals, giving rise to complex spectral signatures. Resonance Raman scattering and surface-enhanced Raman scattering can be used to enhance the signals and reduce the spectral complexity. Raman-active labels can be introduced to increase specificity and multimodality. In addition, nonlinear coherent Raman scattering methods offer higher sensitivities, which enable the rapid imaging of larger sampling areas. Finally, fiber-based imaging techniques pave the way towards in vivo applications of Raman spectroscopy. This Review summarizes the basic principles behind medical Raman imaging and its progress since 2012.

Involved external institutions

Leibniz-Institut für Photonische Technologien e.V. DE Germany (DE) Friedrich-Schiller-Universität Jena DE Germany (DE)

How to cite

APA:

Krafft, C., Schmitt, M., Schie, I.W., Cialla-May, D., Matthaeus, C., Bocklitz, T., & Popp, J. (2017). Label-Free Molecular Imaging of Biological Cells and Tissues by Linear and Nonlinear Raman Spectroscopic Approaches. Angewandte Chemie International Edition, 56(16), 4392-4430. https://doi.org/10.1002/anie.201607604

MLA:

Krafft, Christoph, et al. "Label-Free Molecular Imaging of Biological Cells and Tissues by Linear and Nonlinear Raman Spectroscopic Approaches." Angewandte Chemie International Edition 56.16 (2017): 4392-4430.

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