Heterozygous modulation of TGF-β signaling does not influence Müller glia cell reactivity or proliferation following NMDA-induced damage
Kugler M, Schlecht A, Fuchshofer R, Kleiter I, Aigner L, Tamm ER, Braunger BM (2015)
Publication Type: Journal article
Publication year: 2015
Journal
Book Volume: 144
Pages Range: 443-455
Journal Issue: 5
DOI: 10.1007/s00418-015-1354-y
Abstract
The stimulation of progenitor or stem cells proliferation in the retina could be a therapeutic avenue for the treatment of various ocular neurodegenerative disorders. Müller glia cells have been discussed to represent a progenitor cell population in the adult retina. In the brain, TGF-β signaling regulates the fate of stem cells; however, its role in the vertebrate retina is unclear. We therefore investigated whether manipulation of the TGF-β signaling pathway is sufficient to promote Müller glia cell proliferation and subsequently their trans-differentiation into retinal neurons. To this end, we used mice with heterozygous deficiency of the essential TGF-β receptor type II or of the inhibitory protein SMAD7, in order to down- or up-regulate the activity of TGF-β signaling, respectively. Excitotoxic damage was applied by intravitreal N-methyl-d-aspartate injection, and BrdU pulse experiments were used to label proliferative cells. Although we successfully stimulated Müller glia cell reactivity, our findings indicate that a moderate modulation of TGF-β signaling is not sufficient to provoke Müller glia cell proliferation. Hence, TGF-β signaling in the retina might not be the essential causative factor to maintain mammalian Müller cells in a quiescent, non-proliferative state that prevents a stem cell-like function.
Involved external institutions
Universität Regensburg
Germany (DE)
Ruhr-Universität Bochum (RUB)
Germany (DE)
Paracelsus Medizinische Privatuniversität
Austria (AT)
Germany (DE)
Ruhr-Universität Bochum (RUB)
Germany (DE)
Paracelsus Medizinische Privatuniversität
Austria (AT)
How to cite
APA:
Kugler, M., Schlecht, A., Fuchshofer, R., Kleiter, I., Aigner, L., Tamm, E.R., & Braunger, B.M. (2015). Heterozygous modulation of TGF-β signaling does not influence Müller glia cell reactivity or proliferation following NMDA-induced damage. Histochemistry and Cell Biology, 144(5), 443-455. https://doi.org/10.1007/s00418-015-1354-y
MLA:
Kugler, Martina, et al. "Heterozygous modulation of TGF-β signaling does not influence Müller glia cell reactivity or proliferation following NMDA-induced damage." Histochemistry and Cell Biology 144.5 (2015): 443-455.
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