Cell differentiation defines acute and chronic infection cell types in Staphylococcus aureus

Garcia-Betancur JC, Goni-Moreno A, Horger T, Schott M, Sharan M, Eikmeier J, Wohlmuth B, Zernecke A, Ohlsen K, Kuttler C, Lopez D (2017)

Publication Type: Journal article

Publication year: 2017

Journal

eLife eLife Sciences Publications

Book Volume: 6

Article Number: e28023

DOI: 10.7554/eLife.28023

Abstract

A central question to biology is how pathogenic bacteria initiate acute or chronic infections. Here we describe a genetic program for cell-fate decision in the opportunistic human pathogen Staphylococcus aureus, which generates the phenotypic bifurcation of the cells into two genetically identical but different cell types during the course of an infection. Whereas one cell type promotes the formation of biofilms that contribute to chronic infections, the second type is planktonic and produces the toxins that contribute to acute bacteremia. We identified a bimodal switch in the agr quorum sensing system that antagonistically regulates the differentiation of these two physiologically distinct cell types. We found that extracellular signals affect the behavior of the agr bimodal switch and modify the size of the specialized subpopulations in specific colonization niches. For instance, magnesium-enriched colonization niches causes magnesium binding to S. aureusteichoic acids and increases bacterial cell wall rigidity. This signal triggers a genetic program that ultimately downregulates the agr bimodal switch. Colonization niches with different magnesium concentrations influence the bimodal system activity, which defines a distinct ratio between these subpopulations; this in turn leads to distinct infection outcomes in vitro and in an in vivo murine infection model. Cell differentiation generates physiological heterogeneity in clonal bacterial infections and helps to determine the distinct infection types.

Involved external institutions

Julius-Maximilians-Universität Würzburg DE Germany (DE) Newcastle University GB United Kingdom (GB) Technische Universität München (TUM) DE Germany (DE) Universitätsklinikum Würzburg DE Germany (DE)

How to cite

APA:

Garcia-Betancur, J.-C., Goni-Moreno, A., Horger, T., Schott, M., Sharan, M., Eikmeier, J.,... Lopez, D. (2017). Cell differentiation defines acute and chronic infection cell types in Staphylococcus aureus. eLife, 6. https://doi.org/10.7554/eLife.28023

MLA:

Garcia-Betancur, Juan-Carlos, et al. "Cell differentiation defines acute and chronic infection cell types in Staphylococcus aureus." eLife 6 (2017).

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