Quantification of A. fumiga tu s-specific CD154+ T-cells-preanalytic considerations
Wurster S, Weis P, Page L, Lazariotou M, Einsele H, Ullmann AJ (2017)
Publication Type: Journal article
Publication year: 2017
Journal
Book Volume: 55
Pages Range: 223-227
Journal Issue: 2
DOI: 10.1093/mmy/myw054
Abstract
Fungal specific CD154+ T-cells have been described as a biomarker in invasive aspergillosis. The influence of sample storage on the detection of these cells was assessed. Six-hour delay prior to PBMC isolation is associated with an 18% decrease of cell viability and alterations of the cellular composition of the sample. This results in 87% reduction of CD154+ A. fumigatus specific cells due to reduced assay sensitivity and increased background values in unstimulated samples. If prompt cell measurement is not feasible, isolated PBMCs can be frozen (at -20°C and -80°C) and processed later with comparable assay reliability (mean value fresh vs. thawing: 0.126, 0.133; Pearson-Coefficient: 0.962).
Involved external institutions
Germany (DE)How to cite
APA:
Wurster, S., Weis, P., Page, L., Lazariotou, M., Einsele, H., & Ullmann, A.J. (2017). Quantification of A. fumiga tu s-specific CD154+ T-cells-preanalytic considerations. Medical Mycology, 55(2), 223-227. https://doi.org/10.1093/mmy/myw054
MLA:
Wurster, Sebastian, et al. "Quantification of A. fumiga tu s-specific CD154+ T-cells-preanalytic considerations." Medical Mycology 55.2 (2017): 223-227.
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