Cutting edge: Imbalanced cation homeostasis in MAGT1-deficient B cells dysregulates B cell development and signaling in mice

Gotru SK, Gil-Pulido J, Beyersdorf N, Diefenbach A, Becker IC, Vogtle T, Remer K, Chubanov V, Gudermann T, Hermanns HM, Nieswandt B, Kerkau T, Zernecke A, Braun A (2018)

Publication Type: Journal article

Publication year: 2018

Journal

Journal of Immunology American Association of Immunologists

Book Volume: 200

Pages Range: 2529-2534

Journal Issue: 8

DOI: 10.4049/jimmunol.1701467

Abstract

Cation homeostasis, in relation to various immune-suppressive diseases, is a novel field of investigation. Recently, patients with a loss-of-function mutation in magnesium transporter 1 (MAGT1) were reported to present a dysregulated Mg 2+ homeostasis in T lymphocytes. Using Magt1-knockout mice (Magt1 2/y ), we show that Mg 2+ homeostasis was impaired in Magt1 2/y B cells and Ca 2+ influx was increased after BCR stimulation, whereas T and NK cell function was unaffected. Consequently, mutant B cells displayed an increased phosphorylation of BCR-related proteins differentially affecting protein kinase C activation. These in vitro findings translated into increased frequencies of CD19 + B cells and marginal zone B cells and decreased frequencies of plasma cells among CD45 + splenocytes in vivo. Altogether, our study demonstrates for the first time, to our knowledge, that abolished MAGT1 function causes imbalanced cation homeostasis and developmental responses in B cells. Therefore, this study might contribute to a further understanding of B cell–related pathologies.

Involved external institutions

Universitätsklinikum Würzburg DE Germany (DE) Julius-Maximilians-Universität Würzburg DE Germany (DE) Charité - Universitätsmedizin Berlin DE Germany (DE) Ludwig-Maximilians-Universität (LMU) DE Germany (DE)

How to cite

APA:

Gotru, S.K., Gil-Pulido, J., Beyersdorf, N., Diefenbach, A., Becker, I.C., Vogtle, T.,... Braun, A. (2018). Cutting edge: Imbalanced cation homeostasis in MAGT1-deficient B cells dysregulates B cell development and signaling in mice. Journal of Immunology, 200(8), 2529-2534. https://doi.org/10.4049/jimmunol.1701467

MLA:

Gotru, Sanjeev Kiran, et al. "Cutting edge: Imbalanced cation homeostasis in MAGT1-deficient B cells dysregulates B cell development and signaling in mice." Journal of Immunology 200.8 (2018): 2529-2534.

BibTeX: Download