Spatial and temporal heterogeneity of mouse and human microglia at single-cell resolution
Masuda T, Sankowski R, Staszewski O, Boettcher C, Amann L, Scheiwe C, Nessler S, Kunz P, Van Loo G, Coenen VA, Reinacher PC, Michel A, Sure U, Gold R, Priller J, Stadelmann C, Prinz M (2019)
Publication Type: Journal article
Publication year: 2019
Journal
Book Volume: 566
Pages Range: 388-392
Journal Issue: 7744
DOI: 10.1038/s41586-019-0924-x
Abstract
Microglia have critical roles not only in neural development and homeostasis, but also in neurodegenerative and neuroinflammatory diseases of the central nervous system1–4. These highly diverse and specialized functions may be executed by subsets of microglia that already exist in situ, or by specific subsets of microglia that develop from a homogeneous pool of cells on demand. However, little is known about the presence of spatially and temporally restricted subclasses of microglia in the central nervous system during development or disease. Here we combine massively parallel single-cell analysis, single-molecule fluorescence in situ hybridization, advanced immunohistochemistry and computational modelling to comprehensively characterize subclasses of microglia in multiple regions of the central nervous system during development and disease. Single-cell analysis of tissues of the central nervous system during homeostasis in mice revealed specific time- and region-dependent subtypes of microglia. Demyelinating and neurodegenerative diseases evoked context-dependent subtypes of microglia with distinct molecular hallmarks and diverse cellular kinetics. Corresponding clusters of microglia were also identified in healthy human brains, and the brains of patients with multiple sclerosis. Our data provide insights into the endogenous immune system of the central nervous system during development, homeostasis and disease, and may also provide new targets for the treatment of neurodegenerative and neuroinflammatory pathologies.
Involved external institutions
Albert-Ludwigs-Universität Freiburg
Germany (DE)
Charité - Universitätsmedizin Berlin
Germany (DE)
Universitätsklinikum Göttingen
Germany (DE)
VIB-UGent Center for Inflammation Research
Belgium (BE)
Universitätsklinikum Essen
Germany (DE)
Ruhr-Universität Bochum (RUB)
Germany (DE)
Germany (DE)
Charité - Universitätsmedizin Berlin
Germany (DE)
Universitätsklinikum Göttingen
Germany (DE)
VIB-UGent Center for Inflammation Research
Belgium (BE)
Universitätsklinikum Essen
Germany (DE)
Ruhr-Universität Bochum (RUB)
Germany (DE)
How to cite
APA:
Masuda, T., Sankowski, R., Staszewski, O., Boettcher, C., Amann, L., Scheiwe, C.,... Prinz, M. (2019). Spatial and temporal heterogeneity of mouse and human microglia at single-cell resolution. Nature, 566(7744), 388-392. https://doi.org/10.1038/s41586-019-0924-x
MLA:
Masuda, Takahiro, et al. "Spatial and temporal heterogeneity of mouse and human microglia at single-cell resolution." Nature 566.7744 (2019): 388-392.
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