Single-cell chromatin accessibility landscape identifies tissue repair program in human regulatory T cells
Delacher M, Simon M, Sanderink L, Hotz-Wagenblatt A, Wuttke M, Schambeck K, Schmidleithner L, Bittner S, Pant A, Ritter U, Hehlgans T, Riegel D, Schneider V, Groeber-Becker FK, Eigenberger A, Gebhard C, Strieder N, Fischer A, Rehli M, Hoffmann P, Edinger M, Strowig T, Huehn J, Schmidl C, Werner JM, Prantl L, Brors B, Imbusch CD, Feuerer M (2021)
Publication Type: Journal article
Publication year: 2021
Journal
Book Volume: 54
Pages Range: 702-720.e17
Journal Issue: 4
DOI: 10.1016/j.immuni.2021.03.007
Abstract
Murine regulatory T (Treg) cells in tissues promote tissue homeostasis and regeneration. We sought to identify features that characterize human Treg cells with these functions in healthy tissues. Single-cell chromatin accessibility profiles of murine and human tissue Treg cells defined a conserved, microbiota-independent tissue-repair Treg signature with a prevailing footprint of the transcription factor BATF. This signature, combined with gene expression profiling and TCR fate mapping, identified a population of tissue-like Treg cells in human peripheral blood that expressed BATF, chemokine receptor CCR8 and HLA-DR. Human BATF+CCR8+ Treg cells from normal skin and adipose tissue shared features with nonlymphoid T follicular helper-like (Tfh-like) cells, and induction of a Tfh-like differentiation program in naive human Treg cells partially recapitulated tissue Treg regenerative characteristics, including wound healing potential. Human BATF+CCR8+ Treg cells from healthy tissue share features with tumor-resident Treg cells, highlighting the importance of understanding the context-specific functions of these cells.
Involved external institutions
Regensburg Center for Interventional Immunology
Germany (DE)
Ruprecht-Karls-Universität Heidelberg
Germany (DE)
Deutsches Krebsforschungszentrum (DKFZ)
Germany (DE)
Universitätsklinikum Würzburg
Germany (DE)
Universitätsklinikum Regensburg
Germany (DE)
Helmholtz-Zentrum für Infektionsforschung (HZI)
Germany (DE)
Germany (DE)
Ruprecht-Karls-Universität Heidelberg
Germany (DE)
Deutsches Krebsforschungszentrum (DKFZ)
Germany (DE)
Universitätsklinikum Würzburg
Germany (DE)
Universitätsklinikum Regensburg
Germany (DE)
Helmholtz-Zentrum für Infektionsforschung (HZI)
Germany (DE)
How to cite
APA:
Delacher, M., Simon, M., Sanderink, L., Hotz-Wagenblatt, A., Wuttke, M., Schambeck, K.,... Feuerer, M. (2021). Single-cell chromatin accessibility landscape identifies tissue repair program in human regulatory T cells. Immunity, 54(4), 702-720.e17. https://doi.org/10.1016/j.immuni.2021.03.007
MLA:
Delacher, Michael, et al. "Single-cell chromatin accessibility landscape identifies tissue repair program in human regulatory T cells." Immunity 54.4 (2021): 702-720.e17.
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