Ni(II)-NTA Modified Poly(ethylene imine) Glycopolymers: Physicochemical properties and first in vitro study of polyplexes formed with HIV-derived peptides
Hauptmann N, Pion M, Munoz-Fernandez MA, Komber H, Werner C, Voit B, Appelhans D (2013)
Publication Type: Journal article
Publication year: 2013
Journal
Book Volume: 13
Pages Range: 531-538
Journal Issue: 5
Abstract
Alternative delivery entities are desirable in immunotherapies in which polyplexes are widely formed by electrostatic interactions to induce cellular uptake processes for bioactive molecules. In our study, biocompatible Ni(II)-nitrilo(triacetic acid)-modified poly(ethylene imine)-maltose (Ni-NTA-DG) is realized and evaluated as complexation agent against His-tagged peptides using fluorescence polarization and dynamic light scattering. The polyplexes are stable until a pH of 6.5-6.0, and also up to 50mM of imidazole. A first uptake approach shows that polyplexes lead to an increase in peptide uptake in monocyte-derived immature dendritic cells. In summary, Ni-NTA-DG represents a promising (delivery) platform for forthcoming in vitro applications. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Involved external institutions
Leibniz-Institut für Polymerforschung Dresden e. V.
Germany (DE)
Hospital General Universitario Gregorio Marañón
Spain (ES)
Germany (DE)
Hospital General Universitario Gregorio Marañón
Spain (ES)
How to cite
APA:
Hauptmann, N., Pion, M., Munoz-Fernandez, M.-A., Komber, H., Werner, C., Voit, B., & Appelhans, D. (2013). Ni(II)-NTA Modified Poly(ethylene imine) Glycopolymers: Physicochemical properties and first in vitro study of polyplexes formed with HIV-derived peptides. Macromolecular Bioscience, 13(5), 531-538. https://doi.org/10.1002/mabi.201200449
MLA:
Hauptmann, Nicole, et al. "Ni(II)-NTA Modified Poly(ethylene imine) Glycopolymers: Physicochemical properties and first in vitro study of polyplexes formed with HIV-derived peptides." Macromolecular Bioscience 13.5 (2013): 531-538.
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