Ecotoxicity assessment using ciliate cells in millifluidic droplets
Illing R, Burkart C, Pfitzner D, Jungmann D, Baraban L, Cuniberti G (2016)
Publication Type: Journal article
Publication year: 2016
Journal
Book Volume: 10
Article Number: 024115
Journal Issue: 2
DOI: 10.1063/1.4944869
Abstract
Precise analysis of the aquatic cells and their responses to the toxic chemicals, i.e., water disinfective agents, is of crucial importance due to their role in the ecosystem. We demonstrate the application of the droplets based millifluidic tool for isolating and longtime monitoring of single Paramecium tetraurelia cells using a large number of water-in-oil emulsion droplets. Due to the automated monitoring of the fluorescence signal, the droplets containing cells are distinguished from the empty reservoirs. A viability indicator is used to follow the metabolic dynamic of the cells in every single droplet. Finally, we perform ecotoxicity tests in droplets, exposing the encapsulated paramecia cells to silver nitrate for determination of EC50 levels, and compare the output with the conventional microtiter plate assay.
Involved external institutions
Germany (DE)How to cite
APA:
Illing, R., Burkart, C., Pfitzner, D., Jungmann, D., Baraban, L., & Cuniberti, G. (2016). Ecotoxicity assessment using ciliate cells in millifluidic droplets. Biomicrofluidics, 10(2). https://doi.org/10.1063/1.4944869
MLA:
Illing, Rico, et al. "Ecotoxicity assessment using ciliate cells in millifluidic droplets." Biomicrofluidics 10.2 (2016).
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