Advanced Non-Destructive Ocular Visualization Methods by Improved X-Ray Imaging Techniques
Enders C, Braig EM, Scherer K, Werner JU, Lang GK, Lang GE, Pfeiffer F, Noel P, Rummeny E, Herzen J (2017)
Publication Type: Journal article
Publication year: 2017
Journal
Book Volume: 12
Article Number: e0170633
Journal Issue: 1
DOI: 10.1371/journal.pone.0170633
Abstract
Due to limited X-ray contrast, the use of micro-CT in histology is so far not as widespread as predicted. While specific staining procedures-mostly using iodine-address this shortcoming, long diffusion times restrict its use in the often time-constrained daily routine. Recently, a novel staining protocol has been proposed using a biochemical preconditioning step, which increases the permeability of the cells for the staining agent. This could enable the imaging of entire organs of small mammals at a yet unmatched image quality with reasonable preparation and scan times. We here propose an adaptation of this technique for virtual ophthalmology and histology by volumetrically assessing both human and porcine eyes. Hereby, we demonstrate that (contrast-enhanced) micro-CT can outperform conventional histology in the assessment of tumor entities, as well as functioning as a supplementary tool for surgeons in the positioning of intraocular implants in-vitro and as a general assessment tool for ophthalmologic specimens.
Involved external institutions
Germany (DE)How to cite
APA:
Enders, C., Braig, E.-M., Scherer, K., Werner, J.U., Lang, G.K., Lang, G.E.,... Herzen, J. (2017). Advanced Non-Destructive Ocular Visualization Methods by Improved X-Ray Imaging Techniques. PLoS ONE, 12(1). https://doi.org/10.1371/journal.pone.0170633
MLA:
Enders, Christian, et al. "Advanced Non-Destructive Ocular Visualization Methods by Improved X-Ray Imaging Techniques." PLoS ONE 12.1 (2017).
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