Everolimus-eluting stents stabilize plaque inflammation in vivo: Assessment by intravascular fluorescence molecular imaging

Press MAC, Mallas G, Rosenthal A, Hara T, Mauskapf A, Nudelman RN, Sheehy A, Polyakov IV, Kolodgie F, Virmani R, Guerrero JL, Ntziachristos V, Jaffer FA (2017)

Publication Type: Journal article

Publication year: 2017

Journal

European Heart Journal - Cardiovascular Imaging Oxford University Press

Book Volume: 18

Pages Range: 510-518

Journal Issue: 5

DOI: 10.1093/ehjci/jew228

Abstract

Aims: Inflammation drives atherosclerosis complications and is a promising therapeutic target for plaque stabilization. At present, it is unknown whether local stenting approaches can stabilize plaque inflammation in vivo. Here, we investigate whether everolimus-eluting stents (EES) can locally suppress plaque inflammatory protease activity in vivo using intravascular near-infrared fluorescence (NIRF) molecular imaging. Methods and results: Balloon-injured, hyperlipidaemic rabbits with atherosclerosis received non-overlapping EES and bare metal stents (BMS) placement into the infrarenal aorta (n = 7 EES, n = 7 BMS, 3.5 mm diameter x 12 mm length). Four weeks later, rabbits received an injection of the cysteine protease-activatable NIRF imaging agent Prosense VM110. Twenty-four hours later, co-registered intravascular 2D NIRF, X-ray angiography and intravascular ultrasound imaging were performed. In vivo EES-stented plaques contained substantially reduced NIRF inflammatory protease activity compared with untreated plaques and BMS-stented plaques (P = 0.006). Ex vivo macroscopic NIRF imaging of plaque protease activity corroborated the in vivo results (P = 0.003). Histopathology analyses revealed that EES-treated plaques showed reduced neointimal and medial arterial macrophage and cathepsin B expression compared with unstented and BMS-treated plaques. Conclusions: EES-stenting stabilizes plaque inflammation as assessed by translational intravascular NIRF molecular imaging in vivo. These data further support that EES may provide a local approach for stabilizing inflamed plaques.

Involved external institutions

Harvard University US United States (USA) (US) Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich DE Germany (DE) Abbott Vascular International BVBA US United States (USA) (US) CVPath Institute US United States (USA) (US)

How to cite

APA:

Press, M.A.C., Mallas, G., Rosenthal, A., Hara, T., Mauskapf, A., Nudelman, R.N.,... Jaffer, F.A. (2017). Everolimus-eluting stents stabilize plaque inflammation in vivo: Assessment by intravascular fluorescence molecular imaging. European Heart Journal - Cardiovascular Imaging, 18(5), 510-518. https://doi.org/10.1093/ehjci/jew228

MLA:

Press, Marcella A. Calfon, et al. "Everolimus-eluting stents stabilize plaque inflammation in vivo: Assessment by intravascular fluorescence molecular imaging." European Heart Journal - Cardiovascular Imaging 18.5 (2017): 510-518.

BibTeX: Download