Spectral unmixing techniques for optoacoustic imaging of tissue pathophysiology

Tzoumas S, Ntziachristos V (2017)

Publication Type: Journal article, Review article

Publication year: 2017

Journal

Philosophical Transactions of the Royal Society A-Mathematical Physical and Engineering Sciences Royal Society, The

Book Volume: 375

Article Number: 20170262

Journal Issue: 2107

DOI: 10.1098/rsta.2017.0262

Abstract

A key feature of optoacoustic imaging is the ability to illuminate tissue at multiple wavelengths and therefore record images with a spectral dimension. While optoacoustic images at single wavelengths reveal morphological features, in analogy to ultrasound imaging or X-ray imaging, spectral imaging concedes sensing of intrinsic chromophores and externally administered agents that can reveal physiological, cellular and subcellular functions. Nevertheless, identification of spectral moieties within images obtained at multiple wavelengths requires spectral unmixing techniques, which present a unique mathematical problem given the three-dimensional nature of the optoacoustic images. Herein we discuss progress with spectral unmixing techniques developed for multispectral optoacoustic tomography. We explain how different techniques are required for accurate sensing of intrinsic tissue chromophores such as oxygenated and deoxygenated haemoglobin versus extrinsically administered photo-absorbing agents and nanoparticles. Finally, we review recent developments that allow accurate quantification of blood oxygen saturation (sO2) by transforming and solving the sO2 estimation problem from the spatial to the spectral domain. This article is part of the themed issue ‘Challenges for chemistry in molecular imaging’.

Involved external institutions

Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich DE Germany (DE)

How to cite

APA:

Tzoumas, S., & Ntziachristos, V. (2017). Spectral unmixing techniques for optoacoustic imaging of tissue pathophysiology. Philosophical Transactions of the Royal Society A-Mathematical Physical and Engineering Sciences, 375(2107). https://doi.org/10.1098/rsta.2017.0262

MLA:

Tzoumas, Stratis, and Vasilis Ntziachristos. "Spectral unmixing techniques for optoacoustic imaging of tissue pathophysiology." Philosophical Transactions of the Royal Society A-Mathematical Physical and Engineering Sciences 375.2107 (2017).

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