Increased cell survival and cytogenetic integrity by spatial dose redistribution at a compact synchrotron X-ray source
Burger K, Ilicic K, Dierolf M, Guenther B, Walsh DWM, Schmid E, Eggl E, Achterhold K, Gleich B, Combs SE, Molls M, Schmid TE, Pfeiffer F, Wilkens JJ (2017)
Publication Type: Journal article
Publication year: 2017
Journal
Book Volume: 12
Article Number: e0186005
Journal Issue: 10
DOI: 10.1371/journal.pone.0186005
Abstract
X-ray microbeam radiotherapy can potentially widen the therapeutic window due to a geometrical redistribution of the dose. However, high requirements on photon flux, beam collimation, and system stability restrict its application mainly to large-scale, cost-intensive synchrotron facilities. With a unique laser-based Compact Light Source using inverse Compton scattering, we investigated the translation of this promising radiotherapy technique to a machine of future clinical relevance. We performed in vitro colony-forming assays and chromosome aberration tests in normal tissue cells after microbeam irradiation compared to homogeneous irradiation at the same mean dose using 25 keV X-rays. The microplanar pattern was achieved with a tungsten slit array of 50 μm slit size and a spacing of 350 μm. Applying microbeams significantly increased cell survival for a mean dose above 2 Gy, which indicates fewer normal tissue complications. The observation of significantly less chromosome aberrations suggests a lower risk of second cancer development. Our findings provide valuable insight into the mechanisms of microbeam radiotherapy and prove its applicability at a compact synchrotron, which contributes to its future clinical translation.
Involved external institutions
Germany (DE)How to cite
APA:
Burger, K., Ilicic, K., Dierolf, M., Guenther, B., Walsh, D.W.M., Schmid, E.,... Wilkens, J.J. (2017). Increased cell survival and cytogenetic integrity by spatial dose redistribution at a compact synchrotron X-ray source. PLoS ONE, 12(10). https://doi.org/10.1371/journal.pone.0186005
MLA:
Burger, Karin, et al. "Increased cell survival and cytogenetic integrity by spatial dose redistribution at a compact synchrotron X-ray source." PLoS ONE 12.10 (2017).
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