Cell type atlas and lineage tree of a whole complex animal by single-cell transcriptomics

Plass M, Solana J, Wolf FA, Ayoub S, Misios A, Glazar P, Obermayer B, Theis FJ, Kocks C, Rajewsky N (2018)

Publication Type: Journal article

Publication year: 2018

Journal

Science American Association for the Advancement of Science

Book Volume: 360

Article Number: eaaq1723

Journal Issue: 6391

DOI: 10.1126/science.aaq1723

Abstract

Flatworms of the species Schmidtea mediterranea are immortal—adult animals contain a large pool of pluripotent stem cells that continuously differentiate into all adult cell types. Therefore, single-cell transcriptome profiling of adult animals should reveal mature and progenitor cells. By combining perturbation experiments, gene expression analysis, a computational method that predicts future cell states from transcriptional changes, and a lineage reconstruction method, we placed all major cell types onto a single lineage tree that connects all cells to a single stem cell compartment. We characterized gene expression changes during differentiation and discovered cell types important for regeneration. Our results demonstrate the importance of single-cell transcriptome analysis for mapping and reconstructing fundamental processes of developmental and regenerative biology at high resolution.

Involved external institutions

Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich DE Germany (DE) Max-Delbrück-Centrum für Molekulare Medizin / Max Delbrück Center for Molecular Medicine (MDC) Berlin-Buch DE Germany (DE)

How to cite

APA:

Plass, M., Solana, J., Wolf, F.A., Ayoub, S., Misios, A., Glazar, P.,... Rajewsky, N. (2018). Cell type atlas and lineage tree of a whole complex animal by single-cell transcriptomics. Science, 360(6391). https://doi.org/10.1126/science.aaq1723

MLA:

Plass, Mireya, et al. "Cell type atlas and lineage tree of a whole complex animal by single-cell transcriptomics." Science 360.6391 (2018).

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